Key Product Details
Structure / Form
Ala16-Ser247, with a C-terminal 10-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
|Formulation||Supplied as a 0.2 μm filtered solution in HCl and NaCl.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Trypsin 2/PRSS2
Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).
- Emi, M. et al. (1986) Gene. 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
- Rinderknecht, H. et al. (1979) Gut. 20:886.
Product Specific Notices for Recombinant Human Trypsin 2/PRSS2 Protein, CF
For research use only