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Recombinant Human Trypsin 2/PRSS2 Protein, CF

Catalog # 3586-SE | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Size / Price

Key Product Details

Accession #



Structure / Form

Pro form




Enzyme Activity

Product Specifications


Mouse myeloma cell line, NS0-derived human Trypsin 2/PRSS2 protein
Ala16-Ser247, with a C-terminal 10-His tag


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

26 kDa


34 kDa, reducing conditions


Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in HCl and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Trypsin 2/PRSS2

Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).


  1. Emi, M. et al. (1986) Gene. 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
  3. Rinderknecht, H. et al. (1979) Gut. 20:886.

Alternate Names

PRSS2, Tesp4, TRY8, Trypsinogen 20

Entrez Gene IDs

5645 (Human); 22072 (Mouse); 25052 (Rat)

Gene Symbol



Product Documents for Recombinant Human Trypsin 2/PRSS2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Trypsin 2/PRSS2 Protein, CF

For research use only

Setting the standard in quality research reagents