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Recombinant Human TMEM119 Fc Chimera Protein, CF

Catalog # 10313-TM | R&D Systems, Inc. a Bio-Techne Brand
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10313-TM-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TMEM119 protein
Human TMEM119
(Arg26-Met96)
Accession # Q4V9L6.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg26

Predicted Molecular Mass

34 kDa

SDS-PAGE

37-57 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human TMEM119 Fc Chimera Protein, CF

Recombinant Human TMEM119 Fc Chimera Protein SDS-PAGE

Recombinant Human TMEM119 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human TMEM119 Fc Chimera (Catalog # 10313-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-57 kDa and 70-110 kDa, respectively.

Formulation, Preparation and Storage

10313-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM119

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3).  Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).




References

  1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathol. 36:39.
  4. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.

Long Name

Transmembrane Protein 119

Alternate Names

OBIF

Entrez Gene IDs

338773 (Human); 231633 (Mouse)

Gene Symbol

TMEM119

UniProt

Product Documents for Recombinant Human TMEM119 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TMEM119 Fc Chimera Protein, CF

For research use only

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