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Recombinant Human TLR4 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1478-TR

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1478-TR-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TLR4 protein
Glu24-Lys631, with a C-terminal Ser and 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

70.6 kDa

SDS-PAGE

90-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL.

Formulation, Preparation and Storage

1478-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll‑Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD‑2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL‑1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide‑linked homo‑oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

References

  1. Miyake, K. (2004) Semin. Immunol. 16:11.
  2. Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
  3. Erridge, C. (2010) J. Leukoc. Biol. 87:989.
  4. Medzhitov, R. et al. (1997) Nature 388:394.
  5. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  6. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
  7. Akashi, S. et al. (2000) J. Immunol. 164:3471.
  8. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
  9. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
  10. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
  11. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
  12. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
  13. Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Alternate Names

CD284

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Gene Symbol

TLR4

UniProt

Additional TLR4 Products

Product Documents for Recombinant Human TLR4 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TLR4 Protein, CF

For research use only

Citations for Recombinant Human TLR4 Protein, CF (14)

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Customer Reviews for Recombinant Human TLR4 Protein, CF (2)

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  • Recombinant Human TLR4 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/29/2021
    we use this protein to see interactions with TLR4 antibody, it has a very good affinity towards TLR4 monoclonal antibody in direct ELISA.
    Recombinant Human TLR4 Protein, CF 1478-TR
  • Recombinant Human TLR4 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/28/2020
    Different concentrations of human TLR4-His were used to determine the affinity an a TLR4-specific mAb using the Octet system.
    Recombinant Human TLR4 Protein, CF 1478-TR

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