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Recombinant Human Thrombospondin-2 Protein, CF
Catalog # 1635-T2 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Thrombospondin-2 protein
Gly19-Ile1172, with a C-terminal 10-His tag
Gly19-Ile1172, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly19
Predicted Molecular Mass
129 kDa
SDS-PAGE
170-176 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.
The ED50 for this effect is 0.07-0.7 μg/mL.
Scientific Data Images for Recombinant Human Thrombospondin-2 Protein, CF
Recombinant Human Thrombospondin-2 Protein Bioactivity
Recombinant Human Thrombospondin-2 His-tag (Catalog # 1635-T2) supports the adhesion of SVEC4-10 vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.Formulation, Preparation and Storage
1635-T2
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Thrombospondin-2
Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1‑4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal heparin‑binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88‑90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C-terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin‑binding domain is responsible for the destabilization of focal adhesion sites (8‑10). The heparin‑binding domain also mediates binding to Integrins alpha3 beta1 and alpha6 beta1 on microvascular endothelial cells (EC) and Integrin alpha4 beta1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin‑binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).
References
- Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
- Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
- Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
- Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
- LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
- Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
- Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
- Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
- Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
- Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
- Calzada, M.J. et al. (2004) Circ. Res. 94:462.
- Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
- Noh, Y.-H. et al. (2003) J. Invest. Dermatol. 121:1536.
- Simantov, R. et al. (2005) Matrix Biol. 24:27.
- Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
- Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.
Alternate Names
THBS2, Thrombospondin2, TSP-2
Entrez Gene IDs
7058 (Human)
Gene Symbol
THBS2
UniProt
Product Documents for Recombinant Human Thrombospondin-2 Protein, CF
Product Specific Notices for Recombinant Human Thrombospondin-2 Protein, CF
For research use only
Citations for Recombinant Human Thrombospondin-2 Protein, CF (9)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Thrombospondin-2 Protein, CF include:
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Species: Mouse
Sample Types: In Vivo
Applications: In VivoH Yang et al. (2022-03-28), Multi-scale integrative analyses identify THBS2+ cancer-associated fibroblasts as a key orchestrator promoting aggressiveness in early-stage lung adenocarcinoma Theranostics, 2022-012(7):3104-3130.
PMID: 35547750 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot ControlNMP Cartee et al. (2020-11-24), Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine PLoS ONE, 2020-115(11):e0242376.
PMID: 33232360 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureJF Liu et al. (2020-10-06), Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF-&kappaB activation J Cell Mol Med, 2020-10(0).
PMID: 33021341 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayG Inana et al. (2018-03-13), RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018-016(1):63.
PMID: 29534722 -
Species: Human
Sample Types: Tissue Homogenates
Applications: BioassayF Roudnicky et al. (2018-02-22), Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels Oncogene, 2018-00(0).
PMID: 29467494 -
Species: Rat
Sample Types: Whole Cells
Applications: BioassayS Wang et al. (2017-09-27), Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure PLoS ONE, 2017-012(9):e0185388.
PMID: 28953973 -
Species: Human
Sample Types: Whole Tissue
Applications: BioassayPC Chen et al. (2017-01-25), Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression J Hematol Oncol, 2017-010(1):33.
PMID: 28122633 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayHellbach N et al. (2014-07-02), Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome. Hum Mol Genet, 2014-023(23):6177-90.
PMID: 24990151 -
Sample Types: Protein
Applications: BioassayBenner et al. (2013-04-24), Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013-0497(7449):369-73.
PMID: 23615612
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Customer Reviews for Recombinant Human Thrombospondin-2 Protein, CF (1)
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Application: Immunoassay StandardVerified Customer | Posted 09/23/2019We used this protein for developing an ELISA in combination with mAb (cat. MAB16351)) and pAb (cat. AF1635). This combination works well detecting TSP2 in human serum.
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