Recombinant Human Thrombopoietin (NS0-expressed) Protein
Catalog # 288-TPN | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Ser22-Gly353
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 5-15 ng/mL.
Scientific Data Images for Recombinant Human Thrombopoietin (NS0-expressed) Protein
Recombinant Human Thrombopoietin (NS0-expressed) Protein Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 5-15 ng/mL.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 288-TPN
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 288-TPN/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Thrombopoietin/Tpo
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1 - 3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).
References
- Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
- Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
- Li, J. et al. (1999) Br. J. Haematol. 106:345.
- Bartley, T.D. et al. (1994) Cell 77:1117.
- de Sauvage, F.J. et al. (1994) Nature 369:533.
- Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
- Kaushansky, K. et al. (1994) Nature 369:568.
- Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
- Broudy, V.C. et al. (1995) Blood 85:1719.
- Lok, S.I. et al. (1994) Nature 369:565.
- Chen, J. et al. (1995) Blood 86:4054.
- Oda, A. et al. (1996) Blood 87:4664.
- Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
- Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
- Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
- Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human Thrombopoietin (NS0-expressed) Protein
Product Specific Notices for Recombinant Human Thrombopoietin (NS0-expressed) Protein
For research use only
Citations for Recombinant Human Thrombopoietin (NS0-expressed) Protein (12)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Thrombopoietin (NS0-expressed) Protein include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayKunitomi A et al. (2022-10-17), Improved Sendai viral system for reprogramming to naive pluripotency. Cell Rep Methods, 2022-12(11):100317.
PMID: 36447645 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySudo K et al. (2021-09-03), Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo. Stem Cell Res, 2021-056(0):102531.
PMID: 34509158 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayAerts-Kaya F et al. (2020-01-07), SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential. Biol Blood Marrow Transplant, 2020-026(4):634-642.
PMID: 31917271 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayOhta R et al. (2019-06-16), Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition. J Vis Exp, 2019-00(148).
PMID: 31259914 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNiwa A et al. (2011-07-27), A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011-06(7):e22261.
PMID: 21818303 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayUchida N et al. (2011-05-05), Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther., 2011-018(11):1078-86.
PMID: 21544097 -
Sample Types: N/A
Applications: ELISA (Standard)Schipper HS et al. (2010-06-08), A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-056(0):1320.
PMID: 20530731 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKobune M et al. (2008-06-17), Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-036(9):1121-31.
PMID: 18562079 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayCheli Y et al. (2007-07-06), Transcriptional and epigenetic regulation of the integrin collagen receptor locus ITGA1-PELO-ITGA2. Biochim Biophys Acta, 2007-01769(9):546-58.
PMID: 17669516 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayRajesh (2007-02-01), Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007-025(2):490-9.
PMID: 17284653 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKanaji S et al. (2005-02-08), Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation. Blood, 2005-0105(10):3888-92.
PMID: 15701720 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayManz MG et al. (2002-08-22), Prospective isolation of human clonogenic common myeloid progenitors. Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases, 2002-099(18):11872-7.
PMID: 12193648
There are no citations that match your criteria.
FAQs for Recombinant Human Thrombopoietin (NS0-expressed) Protein
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Q: Why do you sell Thrombopoietin protein from two different expression systems? Under what circumstances would it be preferred to use the NS0-expressed protein?
A: The NS0-expressed protein is necessary for the serum-free megakaryocyte colony stimulating assay (Nadine Weich, Genetics Insititute). In all other assays, it works the same as the Sf-21 derived protein.
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Q: What are the differences between the Recombinant Human Thrombopoietin/Tpo Proteins (Catalog #s 288-TP, 288-TPN, and 288-TPE)?
A: Each of these proteins is produced using a different expression system. 288-TP is Spodoptera frugiperda, Sf21 baculovirus-derived; 288-TPN is mouse myeloma cell line, NS0-derived; and 288-TPE is E. coli-derived.
Additionally, the sequence length also varies. 288-TP and 288-TPN begin at Ser22 and end at Gly353. 288-TPE also begins at Ser22 but is truncated at the C-terminus, ending at Leu195. Truncation of the C-terminus has been shown to enhance the biological activity of Tpo.
Reconstitution Calculator
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