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Recombinant Human Thioredoxin 80 Protein, CF

Catalog # 2329-TX | R&D Systems, Inc. a Bio-Techne Brand
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2329-TX-100

Key Product Details

Source

E. coli

Accession #

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human Thioredoxin-80 protein
Val2-Lys81

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.50 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val2

Predicted Molecular Mass

9 kDa (monomer)

Activity

Measured by its ability to induce IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC) in the presence of IL-2. Pekkari, K. et al. (2001) Blood 97:3184.
The ED50 for this effect is 0.150-1.50 µg/mL in the presence of 8 ng/mL of recombinant human IL-2.

Formulation, Preparation and Storage

2329-TX
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, EDTA and DTT.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-80

Human thioredoxin 80 (Trx80) is a proteolytic cleavage product of a larger precursor termed thioredoxin. Thioredoxin (Trx) ia a 12 - 14 kDa nonglycosylated monomeric 105 amino acid (aa) polypeptide that is synthesized by a wide variety of cells (1 - 3). Trx is a member of the thioredoxin family of proteins that contains a key Cys-X-Tyr-Cys motif that participates in redox reactions (4). Trx is considered the principal disulfide reductase in cells (2, 5). Although it possesses no definitive signal sequence, Trx is secreted and is considered to be the only extracellularly occurring thioredoxin. Trx80, by contrast, is a 10 kDa proteolytic cleavage product of Trx that contains the N-terminal 80 aa residues of Trx (5, 6). This form contains the key CGYC motif found in Trx and shares the same 3-dimensional globular structure as Trx. However, the differences between the molecules are significant. Trx80 lacks Trx’s C-terminus and has no oxidoreductase activity. Trx also circulates as a monomer while Trx80 exists a nondisulfide-linked dimer. Endogenous Trx80 is localized on the plasma membrane of monocytes and macrophages, while Trx is found on the cell surface of various cell types (7). Trx80 also shows significant cytokine activity while Trx has none (5). Cytokine activities attributed to Trx80 include the enhancement of eosinophil ADCC (2), the inducement of monocyte proliferation and maturation to a unique IL-10 secreting phenotype, and the upregulation of monocyte membrane CD14, CD40, CD54/ICAM-1, and CD86/B7 (5, 8). A receptor for Trx80 has not been identified. Human Trx80 is 89% aa identical to mouse and rat Trx80, and 35% aa identical to B. subtilis Trx.

References

  1. Wollman, E.E. et al. (1988) J. Biol. Chem. 263:15506.
  2. Balcewicz-Sablinska, M.K. et al. (1991) J. Immunol. 147:2170.
  3. Yamawaki, H. et al. (2003) Circ. Res. 93:1029.
  4. Rundlof, A.-K. and E. Arner (2004) Antioxid. Redox Signal. 6:41.
  5. Pekkari, K. and A. Holmgren (2004) Antioxid. Redox Signal. 6:53.
  6. Nakamura, H. (2004) Antioxid. Redox Signal. 6:15.
  7. Pekkari, K. et al. (2000) J. Biol. Chem. 275:37474.
  8. Pekkari, K. et al. (2004) Blood 105:1598.

Alternate Names

Thioredoxin80

Entrez Gene IDs

7295 (Human)

Gene Symbol

TXN

UniProt

Product Documents for Recombinant Human Thioredoxin 80 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Thioredoxin 80 Protein, CF

For research use only

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