Recombinant Human TFF2 Protein, CF

Name: Recombinant Human TFF2 Protein, CF
Brand: R&D Systems
SKU: 8290-TF
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8290-TF

Citations (1)

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Key Product Details

Source:	CHO
Accession #:	Q03403
Applications:	Bioactivity

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Product Specifications for Recombinant Human TFF2 Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human TFF2 protein Glu24-Tyr129
Purity	>95%, by SDS-PAGE with silver staining
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu24
Predicted Molecular Mass	12 kDa
SDS-PAGE	17-25 kDa, reducing conditions
Activity	Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF2 can effectively induce ERK1/2 phosphorylation.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8290-TF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF2

Trefoil Factor 2 (TFF2), also known as spasmolytic peptide (SP), is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF2 is an approximately 20 kDa glycosylated peptide that plays an important role in epithelial regeneration and wound healing (1, 2). Mature human TFF2 shares 87% and 83% amino acid sequence identity with mouse and rat TFF2, respectively. TFF2 is primarily expressed by gastric mucosa of the pyloric stomach where it binds to Gastrokine 2 (3, 4). It is up-regulated in bronchiolar epithelium following exposure to allergens and is excreted into the urine at increased levels in patients with kidney stones (5, 6). TFF2 knockout mice exhibit increased gastric epithelium damage following H. pylori infection or treatment with non-steroidal anti-inflammatory drugs (7, 8). Administration of TFF2 can reduce the severity of experimental colitis (9-11). TFF2 is down-regulated in many gastric cancers, although it is up-regulated in some breast cancers (12-14). TFF2 promotes the migration of normal epithelial cells as well as tumor cells (14, 15).

Item	Value
References	Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350. May, F.E.B. et al. (2000) Gut 46:454. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505. Otto, W.R. et al. (2006) Proteomics 6:4235. Nikolaidis, N.M. et al. (2006) Exp. Lung Res. 32:483. Rinnert, M. et al. (2010) Cell Tissue Res. 339:639. Fox, J.G. et al. (2007) Am. J. Pathol. 171:1520. Farrell, J.J. et al. (2002) J. Clin. Invest. 109:193. Soriano-Izquierdo, A. et al. (2004) J. Leukoc. Biol. 75:214. Poulsen, S.S. et al. (2005) Regul. Pept. 126:163. Tran, C.P. et al. (1999) Gut 44:636. Leung, W.K. et al. (2002) J. Pathol. 197:582. Kirikoshi, H. and M. Katoh (2002) Int. J. Oncol. 21:655. May, F.E. et al. (2004) Peptides 25:865. Oertel, M. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:418.
Long Name	Trefoil Factor 2
Entrez Gene IDs	7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names	SML1, SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1, SP, Spasmolysin, Spasmolytic Polypeptide, TFF2, trefoil factor 2

Citations for Recombinant Human TFF2 Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TFF2 Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

G Wang et al. (2020), Dexmedetomidine alleviates sleep-restriction-mediated exaggeration of postoperative immunosuppression via splenic TFF2 in aged mice Aging (Albany NY), 12(6):5318-5335.
PMID: 32200357

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Recombinant Human TFF2 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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