Product Specifications for Recombinant Human TC-PTP (aa 2-314) Protein, CF
E. coli-derived human TC-PTP protein Thr2-Asn314, with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
38 kDa, reducing conditions
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006). The specific activity is >30 μmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol and Betamercaptoethanol.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).
Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
T Cell Protein Tyrosine Phosphatase
Entrez Gene IDs
5771 (Human); 19255 (Mouse)
PTP2, PTPN2, PTPTPTN2, T-cell protein tyrosine phosphatase, T-cell protein-tyrosine phosphatase, TC-PTP, TCELLPTP, TCPTP, TCPTPEC 220.127.116.11, protein tyrosine phosphatase, non-receptor type 2, tyrosine-protein phosphatase non-receptor type 2
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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