Recombinant Human Syndecan-4 Protein, CF

Name: Recombinant Human Syndecan-4 Protein, CF
Brand: R&D Systems
SKU: 2918-SD
Rating: 5 (1 reviews)

Catalog # 2918-SD | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (5)

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P31431
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Syndecan-4 Protein, CF

Technical Data
Citations (5) / Reviews (1)

Product Specifications for Recombinant Human Syndecan-4 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Syndecan-4 protein Glu19-Glu145, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu19
Predicted Molecular Mass	14.7 kDa
SDS-PAGE	14.7 kDa
Activity	Measured by its binding ability in a functional ELISA. Immobilized rhSyndecan-4 at 500 ng/mL (100 µL/well) can bind rhFGF-basic with a linear range of 0.1-10 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2918-SD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syndecan-4

Syndecan-4, previously known as amphiglycan or ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20 - 80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present
FGF-2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6 - 8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

Item	Value
References	Tkachenko, E. et al. (2005) Circ. Res. 96:488. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181. David, G. et al. (1992) J. Cell Biol. 118:961. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. Charnaux, N. et al. (2005) FEBS J. 272:1937. Schmidt, A. et al., J. Biol. Chem. 280:34441. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.
Entrez Gene IDs	6385 (Human); 20971 (Mouse)
Alternate Names	Amphiglycan, MGC22217, Ryudocan, Ryudocan core protein, SDC4, SYND4ryudocan amphiglycan, Syndecan-4, Syndecan4, syndecan 4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4

Citations for Recombinant Human Syndecan-4 Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Syndecan-4 Protein, CF include:

Species: Human

Applications: Bioassay

Matsunuma et al. (2023-06-27), Chain-specificity of laminin ?1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding Scientific reports
PMID: 37369727
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

A Hudák et al. (2022-01-12), Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission International Journal of Molecular Sciences, 2022-023(2).
PMID: 35054983
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Hudák et al. (2021-05-19), Contribution of Syndecans to the Cellular Entry of SARS-CoV-2 International Journal of Molecular Sciences, 2021-022(10).
PMID: 34069441
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Parsi MK et al. (2010-04-09), LTBP-2 has multiple heparin/heparan sulfate binding sites. Matrix Biol., 2010-029(5):393-401.
PMID: 20382221
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Wu ZL et al. (2010-02-10), A versatile polyacrylamide gel electrophoresis based sulfotransferase assay. BMC Biotechnol., 2010-010(0):11.
PMID: 20146816

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Recombinant Human Syndecan-4 Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 09/14/2017

Visualized using Simply Blue Safe Stain. Result from blot suggests glycosylation of the syndecan-4 protein.

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Product Documents for Recombinant Human Syndecan-4 Protein, CF

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Recombinant Human Syndecan-4 Protein, CF

Catalog # 2918-SD | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human Syndecan-4 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Syndecan-4

Citations for Recombinant Human Syndecan-4 Protein, CF (5)

Customer Reviews for Recombinant Human Syndecan-4 Protein, CF (1)

Customer Images

Recombinant Human Syndecan-4 Protein, CF

Product Documents for Recombinant Human Syndecan-4 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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