Product Specifications for Recombinant Human Stabilin-2 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human Stabilin-2 protein Ser20-Thr2458, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
260-300 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human Stabilin-2 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.
Scientific Data Examples for Recombinant Human Stabilin-2 His-tag Protein, CF
Recombinant Human Stabilin‑2 His-tag Protein Binding Activity.
When Recombinant Human Stabilin-2 His-tag Protein (Catalog # 11086-S2) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.
Recombinant Human Stabilin‑2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human Stabilin‑2 His-tag Protein (Catalog # 11086-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 260-300 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stabilin-2, also known as HARE (Hyaluronic Acid Receptor for Endocytosis) and FEEL2 (Fasciclin, EGF-like, laminin type EGF-like, and Link domain containing scavenger receptor 2), is a type I transmembrane multidomain protein that is the closest homolog of stabilin1. It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrincoated pits (4). Stabilin2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation endproduct (AGE), collagen Nterminal propeptides and acetylated LDL (4-7). Stabilin-2 has been found to regulate the cancer progression, and high expression of Stabilin-2 was reported to predict poor tumor prognosis in NSCLC (8).
Politz, O. et al. (2002) Biochem. J. 362:155.
Harris, E.N. and F. Cabral (2019) Biomolecules 9:273.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
Yong, J. et al. (2021) Bioengineered 12:3426.
Entrez Gene IDs
55576 (Human); 192188 (Mouse); 282580 (Rat)
CD44-like precursor FELL, DKFZP434E0321, FAS1 EGF-like and X-link domain containing adhesion molecule-2, FAS1 EGF-like and X-link domain-containing adhesion molecule 2, FEEL-2, FEEL2, FELE-2, FELL, FELL-2, FEX2, Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2, HARE, HAREFELL2, Hyaluronan receptor for endocytosis, STAB-2, STAB2, Stabilin-2, Stabilin2, fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2, hepatic hyaluronan clearance receptor, hyaluronic acid receptor for endocytosis, stabilin 2
Citations for Recombinant Human Stabilin-2 His-tag Protein, CF
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Product Documents for Recombinant Human Stabilin-2 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics