Product Specifications for Recombinant Human ST7/LRP12 Protein, CF
Mouse myeloma cell line, NS0-derived human ST7/LRP12 protein Asn28-Ile488 with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Asn28 & Glu33
Predicted Molecular Mass
71 kDa, reducing conditions
Measured by its binding ability in a functional ELISA.
When rhST7 is immobilized at 0.5 µg/mL, the concentration of rhLRPAP (Catalog # 4296-LR) that produces 50% of the optimal binding response is found to be approximately 0.6‑3 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
ST7 (Suppressor of Tumorigenicity 7), also known as RAY1, TSG7 and FAM4A1, is a type I transmembrane protein belonging to the LDLR superfamily and is designated LRP12 (1 - 3). The human ST7 cDNA encodes 859 amino acids (aa) including a 32 aa signal sequence, a 460 aa extracellular domain (ECD) containing two CUB domains and five LDLR class A domains, a 21 aa transmembrane domain, and a 346 aa cytoplasmic domain containing motifs implicated in endocytosis and signal transduction (1, 2). Human ST7 shares 95% aa sequence homology with mouse and rat, 96% with canine, and 98% with bovine, equine and porcine ST7 within the ECD. Genomic sequencing indicates the possibility of up to 18 splicing isoforms, but expression of these has not been well‑studied (3). ST7 is widely expressed in normal tissues, especially fibroblasts (1, 4). Highest mRNA levels were detected in heart and skeletal muscle (1). ST7 was originally proposed to be a tumor suppressor protein, but it is not consistently downregulated in a variety of cancers, either by mutation or loss of heterozygosity (1, 4 - 7). In certain cancers, expression may even be upregulated (8). Expression may be associated with downregulated expression of extracellular matrix molecules that are involved in remodeling, such as SPARC, IGFBP5 and several matrix metalloproteinases, and modulation of in vivo tumorigenicity (4, 5).
Qing, J. et al. (1999) Oncogene 18:335.
Battle, M.A. et al. (2003) Biochemistry 42:7270.
Vincent, J.B. et al. (2002) Genomics 80:283.
Zenclusen, J.C. et al. (2001) Nat. Genet. 27:392.
Hooi, C.F. et al. (2006) Oncogene 25:3924.
Sivasundaram, K. et al. (2003) Oncol. Rep. 10:1737.
Dong, S.M. and D. Sidransky (2002) Clin. Cancer Res. 8:2939.
Garnis, C. et al. (2004) Oncogene 23:2582.
Low Density Lipoprotein Receptor-related 12
Entrez Gene IDs
29967 (Human); 239393 (Mouse)
DKFZp762O2113, FAM4A1, LRP12, Protein FAM4A1, Protein HELG, RAY1, SEN4, ST7, TSG7, subfamily A, member 1, suppression of tumorigenicity 7, suppression of tumorigenicity 7 (breast), suppressor of tumorigenicity 7 protein
Citations for Recombinant Human ST7/LRP12 Protein, CF
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FAQs for Recombinant Human ST7/LRP12 Protein, CF
Why does the Recombinant Human ST7/LRP12 Protein, CF (Catalog # 2560-S7) contain two N-terminal sequences (Asn28 & Glu33)?
The reason for the mixture of two different sequences is most likely due to the presence of splice variants and/or cleavage within the signal peptide sequence, although this has not been confirmed. Accession # Q9Y561 in UniProt shows that the signal peptide sequence Human ST7/LRP12 is aa 1-32 and the mature protein is aa 33-859. Thus, the predominant form of the 2560-S7protein will have an N-terminal sequence of Glu33, and the second form that was cleaved within the signal peptide sequence will have an N-terminal sequence of Asn28.
Product Documents for Recombinant Human ST7/LRP12 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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