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Recombinant Human ST3GAL4 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10496-GT

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10496-GT-020

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST3GAL4 protein
Glu41-Phe333, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

34 kDa

SDS-PAGE

39-41 kDa, under reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to alpha-lactose.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

10496-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST3GAL4

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL4 is an alpha2,3-sialyltransferase and forms alpha2-3 linked sialic acid on the Gal residue of the terminal Gal beta1-4GlcNAc and Gal beta1-3GalNAc structures found on glycoproteins and glycolipids (2, 3). ST3GAL4 is involved in the synthesis of sialyl Lewis x (sLex) glycan epitope that may serve as ligands for E- and P-selectins on activated endothelial cells in inflammatory responses (4). Down-regulation of ST3GAL4 mRNA may be one of the factors associated with the malignant progression of human renal cell carcinoma (5). ST3GAL4 is expressed as a type II membrane protein localized in the trans-Golgi apparatus but may also be proteolytically processed to form a soluble form. The activity of the recombinant human ST3GAL4 was determined using a phosphatase‑coupled glycosyltransferase assay (6).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Kitagawa, H. and Paulson, J.C. (1994) J Biol Chem. 269:1394.
  3. Basu, S.S. et al. (1996) Biochemistry 35:5166.
  4. Higai, K. et al. (2006) FEBS Letters 580:1873.
  5. Saito, S. et al. (2002) Oncology reports  9:1251.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 4

Alternate Names

CGS23, EC 2.4.99.9, Gal-NAc 6S, NANTA3, SAT-3, SIAT4, SIAT4C, ST-4, ST3Gal IV

Entrez Gene IDs

6484 (Human); 20443 (Mouse); 363040 (Rat)

Gene Symbol

ST3GAL4

UniProt

Additional ST3GAL4 Products

Product Documents for Recombinant Human ST3GAL4 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ST3GAL4 His-tag Protein, CF

For research use only

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