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Recombinant Human ST3GAL3 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10554-GT

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10554-GT-020

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ST3GAL3 protein
Lys29-Ile375, with a N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

34 kDa

SDS-PAGE

37-43 kDa, under reducing conditions.

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine.
The specific activity is > 400 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human ST3GAL3 His-tag Protein, CF

Recombinant Human ST3GAL3 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human ST3GAL3 His-tag Protein (Catalog # 10554-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-43 kDa.

Formulation, Preparation and Storage

10554-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST3GAL3

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL3 is an alpha2,3-sialyltransferase and forms alpha2-3 linked sialic acid on the Gal residue of the terminal Gal beta1-4GlcNAc, Gal beta1-3GlcNAc and Gal beta1-3GalNAc structures found on glycoproteins and glycolipids (2). ST3GAL3 is involved in the synthesis of both sialyl Lewis x (sLex), and sialyl Lewis a (sLea) epitopes that can serve as the ligands for E- and P-selectins on activated endothelial cells in inflammation (3). The glycan epitopes formed by ST3GAL3 are a prerequisite for attaining and/or maintaining higher cognitive functions (4) and mutations in this gene have been associated with a form of autosomal recessive nonsyndromic cognitive disability as well as infantile epileptic encephalopathy (5). The enzyme is expressed as a type II membrane protein localized in the trans-Golgi apparatus but can also be proteolytically processed to form a soluble form (6). The activity of the recombinant human ST3GAL3 was determined using a phosphatase‑coupled glycosyltransferase assay (7).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Weinstein, J. et al. (1982) J Biol Chem.  257:13845.
  3. Grahn, A. et al. (2002) Glycoconjugate Journal 19:197.
  4. Hu, H. et al. (2011) Am J Hum Genet. 89:407.
  5. Joshi, H.J. et al. (2018) Glycobiology 28: 284.
  6. Burger, P. C. et al. (1998) Glycobiology 8:245.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3

Alternate Names

MRT12, Siat3, SIAT6, ST3 beta-Gal alpha-2,3-Sialyltransferase 3, St3Gal-III, ST3GalIII, ST3N

Entrez Gene IDs

6487 (Human); 20441 (Mouse); 64445 (Rat)

Gene Symbol

ST3GAL3

UniProt

Additional ST3GAL3 Products

Product Documents for Recombinant Human ST3GAL3 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ST3GAL3 His-tag Protein, CF

For research use only

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