Product Specifications for Recombinant Human ST3GAL3 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived human ST3GAL3 protein Lys29-Ile375, with a N-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
37-43 kDa, under reducing conditions.
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is > 400 pmol/min/μg, as measured under the described conditions.
Scientific Data Examples for Recombinant Human ST3GAL3 His-tag Protein, CF
Recombinant Human ST3GAL3 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human ST3GAL3 His-tag Protein (Catalog # 10554-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-43 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). ST3GAL3 is an alpha 2,3-sialyltransferase and forms alpha 2-3 linked sialic acid on the Gal residue of the terminal Gal beta 1-4GlcNAc, Gal beta 1-3GlcNAc and Gal beta 1-3GalNAc structures found on glycoproteins and glycolipids (2). ST3GAL3 is involved in the synthesis of both sialyl Lewis x (sLex), and sialyl Lewis a (sLea) epitopes that can serve as the ligands for E- and P-selectins on activated endothelial cells in inflammation (3). The glycan epitopes formed by ST3GAL3 are a prerequisite for attaining and/or maintaining higher cognitive functions (4) and mutations in this gene have been associated with a form of autosomal recessive nonsyndromic cognitive disability as well as infantile epileptic encephalopathy (5). The enzyme is expressed as a type II membrane protein localized in the trans-Golgi apparatus but can also be proteolytically processed to form a soluble form (6). The activity of the recombinant human ST3GAL3 was determined using a phosphatase‑coupled glycosyltransferase assay (7).
Varki, A. (1999) Glycobiology 2:25.
Weinstein, J. et al. (1982) J Biol Chem. 257:13845.
Grahn, A. et al. (2002) Glycoconjugate Journal 19:197.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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