Product Specifications for Recombinant Human SOST/Sclerostin His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human SOST/Sclerostin protein Gln24-Tyr213, with a N-terminal 7-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
His of Tag
Predicted Molecular Mass
25-35 kDa, under reducing conditions.
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1.50-6.00 μg/mL.
Scientific Data Examples for Recombinant Human SOST/Sclerostin His-tag Protein, CF
Recombinant Human SOST/Sclerostin His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human SOST/Sclerostin His-tag Protein (Catalog # 10962-ST) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualizedby Coomassie® Blue staining, showing bands at 25-35 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Sclerostin (SOST) is a member of the Cerberus/differential screening-selected gene in neuroblastoma (DAN) family, a group of secreted glycoproteins characterized by a cysteine-knot motif. The Cerberus/DAN family consists of multiple members originally identified as putative BMP antagonists including Dan, SOST, Cerberus, Gremlin, USAG-1, PRDC, and Coco (1, 2). While the overall sequence identity between family members is low, they have conserved spacing of six cysteine residues in the C-terminus which form the structurally conserved cysteine-knot motif. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer (1). Mature human SOST shares 90% amino acid sequence identity with mouse and rat SOST. SOST appears to be the first example of a BMP antagonist predominately localized to osteoclasts and functions as an important regulator of bone homeostasis by inhibiting bone mineralization (3). SOST has been shown to have unique ligand specificity by binding directly to BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity thereby repressing BMP-induced osteogenesis (4). Mutations in the SOST gene can cause several bone dysplasia disorders characterized by progressive skeletal overgrowth including sclerosteosis and van Buchem disease (5). Additionally, SOST has been shown to be a strong Wnt antagonist by directly binding the Wnt co‑receptors LRP5 and LRP6 (6).
Nolan, K. and Thompson, T.B. (2014) Protein Sci. 23:999.
Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
van Bezooijen, R.L. et al. (2004) J. Exp. Med. 199:805.
Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Kim, J. et al. (2020) Nat Commun 11:5357.
Entrez Gene IDs
50964 (Human); 74499 (Mouse)
SOST, VBCHsclerosteosis, sclerostin
Citations for Recombinant Human SOST/Sclerostin His-tag Protein, CF
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Product Documents for Recombinant Human SOST/Sclerostin His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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