Recombinant Human Siglec-9 Fc Chimera Protein, CF

Name: Recombinant Human Siglec-9 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1139-SL
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1139-SL

Citations (10)

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Key Product Details

Source:	NS0
Accession #:	Q9Y336
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Siglec-9 Fc Chimera Protein, CF

Product Specifications for Recombinant Human Siglec-9 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Siglec-9 protein

Human Siglec-9 (Gln18-Gly348) Accession # Q9Y336	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln18 predicted

Predicted Molecular Mass

62.7 kDa (monomer)

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED₅₀ for this effect is 10.0-100 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1139-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

Item	Value
References	Crocker, P.R. et al. (1998) Glycobiology 8:v. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337. Crocker, P.R. and A. Varki (2001) Immunology 103:137. Angata, T. et al. (2002) J. Biol. Chem. 277:24466. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.
Long Name	Sialic Acid Binding Ig-like Lectin 9
Entrez Gene IDs	27180 (Human); 102118310 (Cynomolgus Monkey)
Alternate Names	CD329, CDw329, FOAP-9, OBBP-Like, Protein FOAP-9, Siglec-9, Siglec9, sialic acid binding Ig-like lectin 9, sialic acid-binding Ig-like lectin 9

Citations for Recombinant Human Siglec-9 Fc Chimera Protein, CF (10)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Siglec-9 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Control

OS Adeniji et al. (2021-01-01), Siglec-9 defines and restrains a natural killer subpopulation highly cytotoxic to HIV-infected cells PloS Pathogens, 17(11):e1010034.
PMID: 34762717
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Q Haas et al. (2019-01-01), Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment Cancer Immunol Res, 0(0).
PMID: 30988027
Species: Human
Sample Types: Natural Protein
Applications: Bioassay

J Terävä et al. (2019-01-01), Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 14(7):e0219480.
PMID: 31344060
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

X Wang et al. (2017-01-01), Siglec-9 is upregulated in rheumatoid arthritis and suppresses collagen-induced arthritis through reciprocal regulation of Th17/Treg cell differentiation Scand. J. Immunol, 0(0).
PMID: 28273363
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Zeng et al. (2017-01-01), Increased expression of Siglec-9 in chronic obstructive pulmonary disease Sci Rep, 7(1):10116.
PMID: 28860481
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

T Ito et al. (2017-01-01), Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity Sci Rep, 7(0):44043.
PMID: 28272428
Species: Human
Sample Types: In Vivo
Applications: In Vivo

Takuya Matsumoto (2016-01-01), Soluble Siglec-9 suppresses arthritis in a collagen-induced arthritis mouse model and inhibits M1 activation of RAW264.7 macrophages Arthritis Res Ther, 18(1):133.
PMID: 27267914
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Matsubara K et al. (2015-01-01), Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci, 35(6):2452-64.
PMID: 25673840
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

Chen et al. (2014-01-01), Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1. Elife, 3(0):e04066.
PMID: 25187624
Species: Virus
Sample Types: Virus
Applications: Surface Plasmon Resonance

Zou Z et al. (2011-01-01), Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE, 6(9):e24559.
PMID: 21931755

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Recombinant Human Siglec-9 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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