Product Specifications for Recombinant Human Siglec-15 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein Phe20-Tyr251, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
27-37 kDa, under reducing conditions
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.2-2 μg/mL.
Scientific Data Examples for Recombinant Human Siglec-15 His-tag Protein, CF
Recombinant Human Siglec-15 His-tag Protein Bioactivity.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.2‑2 μg/mL
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Sialic acid-binding Ig-like lectin 15 (Siglec-15) is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Siglecs can be divided into 2 classes: evolutionarily conserved Siglecs and CD33-related Siglecs (2). Siglec-15 belongs to the evolutionarily conserved class and is the only Siglec highly conserved throughout vertebrate evolution (3). Mature human Siglec-15 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% and 84% amino acid sequence identity with mouse and rat Siglec‑15, respectively. Alternative splicing of Siglec-15 generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 is expressed on osteoclasts, macrophages, and dendritic cells (3-7) and binds to the sialyl-Tn antigen (3, 4, 7). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (5-7) and for the production of TGF-beta by tumor-associated macrophages (4). Additionally, Siglec-15 associates with the signaling molecules DAP12 and DAP10 through a lysine residue located in the transmembrane domain (3-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (8). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (8).
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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