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Recombinant Human Siglec-15 His-tag Protein, CF

Catalog # 10698-SL | R&D Systems, Inc. a Bio-Techne Brand
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10698-SL-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein
Phe20-Tyr251, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20

Predicted Molecular Mass

26 kDa

SDS-PAGE

27-37 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.2-2 μg/mL.

Scientific Data Images for Recombinant Human Siglec-15 His-tag Protein, CF

Recombinant Human Siglec-15 His-tag Protein Bioactivity.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.2‑2 μg/mL

Formulation, Preparation and Storage

10698-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Sialic acid-binding Ig-like lectin 15 (Siglec-15) is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Siglecs can be divided into 2 classes: evolutionarily conserved Siglecs and CD33-related Siglecs (2). Siglec-15 belongs to the evolutionarily conserved class and is the only Siglec highly conserved throughout vertebrate evolution (3). Mature human Siglec-15 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% and 84% amino acid sequence identity with mouse and rat Siglec‑15, respectively. Alternative splicing of Siglec-15 generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 is expressed on osteoclasts, macrophages, and dendritic cells (3-7) and binds to the sialyl-Tn antigen (3, 4, 7). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (5-7) and for the production of TGF-beta by tumor-associated macrophages (4). Additionally, Siglec-15 associates with the signaling molecules DAP12 and DAP10 through a lysine residue located in the transmembrane domain (3-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (8). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (8).

References

  1. Humphrey, M.B. and M.C. Nakamura (2016) Clin. Rev. Allergy Immunol. 51:48.
  2. Varki, A. and Angata, T. (2006) Glycobiology, 16:1R.
  3. Angata, T. et al. (2007) Glycobiology 17:838.
  4. Takamiya, R. et al. (2013) Glycobiology 23:178.
  5. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  6. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  7. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
  8. Wang, J. et al. (2019) Nat. med. 25:656.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Gene Symbol

SIGLEC15

UniProt

Product Documents for Recombinant Human Siglec-15 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Siglec-15 His-tag Protein, CF

For research use only

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