Recombinant Human Siglec-15 Fc Chimera Protein, CF

Name: Recombinant Human Siglec-15 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9227-SL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9227-SL

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Key Product Details

Source:	CHO
Accession #:	Q6ZMC9
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Product Specifications for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein

Human Siglec-15 (Phe20-Thr263) Accession # Q6ZMC9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe20

Predicted Molecular Mass

53 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-10 μg/mL.

Scientific Data Examples for Recombinant Human Siglec-15 Fc Chimera Protein, CF

	Recombinant Human Siglec-15 Fc Chimera Protein Bioactivity Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-10 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9227-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

Item	Value
References	Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914. Angata, T. et al. (2007) Glycobiology 17:838. Takamiya, R. et al. (2013) Glycobiology 23:178. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Long Name	Sialic Acid Binding Ig-like Lectin 15
Entrez Gene IDs	284266 (Human); 620235 (Mouse); 498888 (Rat)
Alternate Names	CD33 antigen-like 3SIGLEC-15, CD33 molecule-like 3, CD33L3, HsT1361, Siglec-15, Siglec15, sialic acid binding Ig-like lectin 15, sialic acid-binding Ig-like lectin 15

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Recombinant Human Siglec-15 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: SDS-PAGE Control

Verified Customer | Posted 06/17/2019

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Product Documents for Recombinant Human Siglec-15 Fc Chimera Protein, CF

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Recombinant Human Siglec-15 Fc Chimera Protein, CF

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Product Specifications for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Siglec-15

Citations for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human Siglec-15 Fc Chimera Protein, CF (1)

Recombinant Human Siglec-15 Fc Chimera Protein, CF

Product Documents for Recombinant Human Siglec-15 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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