Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human sFRP-4 Protein

Catalog # 1827-SF | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
Carrier Free
1827-SF-025/CF

Catalog #
Availability
Size / Price
Qty
With Carrier
1827-SF-025

Key Product Details

Source

NS0

Accession #

Structure / Form

Recombinant Human sFRP-4 is prone to proteolytic cleavage at C-terminus, therefore the poly-His tag at C-terminus may not be present in the preparation.

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human sFRP-4 protein
Ala22-Val346, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

38.9 kDa

SDS-PAGE

55 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 4-20 µg/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1827-SF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1827-SF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-4

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the Frizzled proteins, including 10 conserved cysteines; and a 175 aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPs are thought to act as soluble antagonists of Wnt signals, often postulated to act as tumor suppressor genes (1).

sFRP-4, also known as DDC-4, FrpAP, frpHE and FrzB-2, is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.

References

  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
  7. Wong, S.C.C. et al. (2002) J. Pathology 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Entrez Gene IDs

6424 (Human); 20379 (Mouse); 89803 (Rat)

Gene Symbol

SFRP4

UniProt

Additional sFRP-4 Products

Product Documents for Recombinant Human sFRP-4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human sFRP-4 Protein

For research use only

Citations for Recombinant Human sFRP-4 Protein (9)

Showing  1 - 9 of 9 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human sFRP-4 Protein include:

There are no citations that match your criteria.

Showing  1 - 9 of 9 publications Showing All

Customer Reviews for Recombinant Human sFRP-4 Protein (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%
Showing  1 - 1 of 1 review Showing All
Filter By:
  • Recombinant Human sFRP-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/03/2016

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents