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Recombinant Human sFRP-1 (Histidine-tagged) Protein

Catalog # 1384-SF | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1384-SF-025/CF

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With Carrier
1384-SF-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Ser32-Lys314 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser32 & Phe39 & Asp42

Predicted Molecular Mass

33 kDa

SDS-PAGE

35-40 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280.
The ED50 for this effect is 2.5-10 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1384-SF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1384-SF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is upregulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

References

  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.

Long Name

Secreted Frizzled Related Protein 1

Alternate Names

FrzA, SARP-2, sFRP1

Entrez Gene IDs

6422 (Human); 20377 (Mouse); 84402 (Rat)

Gene Symbol

SFRP1

UniProt

Product Documents for Recombinant Human sFRP-1 (Histidine-tagged) Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human sFRP-1 (Histidine-tagged) Protein

For research use only

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