Source	Chinese Hamster Ovary cell line, CHO-derived human SF20/MYDGF protein Val32-Leu173
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val32
Predicted Molecular Mass	16 kDa
SDS-PAGE	14-15 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human SF20/MYDGF (Catalog # 10231-MY) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED₅₀ of 0.8-4.8 μg/mL.

Recombinant Human SF20/MYDGF Protein, CF Scientific Data Examples

	Recombinant Human SF20/MYDGF Protein Binding Activity When Recombinant Human SF20/MYDGF (Catalog # 10231-MY) is immobilized at 2 µg/mL, 100 µg/well, Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED₅₀ of 0.8-4.8 µg/mL.
	Recombinant Human SF20/MYDGF Protein SDS-PAGE 2 μg/lane of Recombinant Human SF20/MYDGF (Catalog # 10231-MY) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-15 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10231-MY

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Human MYDGF shares 92% and 85% amino acid sequence identity to mouse and rat MYDGF, respectively. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

Item	Value
References	Korf-Klingebiel, M. et al. (2015) Nat. Med. 10:3778. Wang, P. et al. (2004) Cell. Mol. Life Sci. 61:2405 Sunagozaka, H. et al. (2011) Int. J. Cancer 129:1576 Bortnov, V. et al. (2018) J. Biol. Chem. 293:13166.
Long Name	Stromal Cell-derived Growth Factor SF20
Entrez Gene IDs	56005 (Human); 28106 (Mouse)
Alternate Names	C19orf10, EUROIMAGE1875335, IL25, IL27, IL27w, Ly6elg, MYDGF, R33729_1, SF20, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10, chromosome 19 open reading frame 10, interleukin 27 working designation, interleukin-25, myeloid-derived growth factor

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Recombinant Human SF20/MYDGF Protein, CF

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