Chinese Hamster Ovary cell line, CHO-derived human Serpin G1/C1 Inhibitor protein Asn23-Ala500 with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
89-98 kDa, under reducing conditions
Measured by its ability to inhibit Recombinant Human Complement Component C1s (Catalog # 2060-SE) cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is <5 nM, as measured under the described conditions.
Recombinant Human Serpin G1 His-tag Protein, CF Scientific Data Examples
Recombinant Human Serpin G1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human Serpin G1 His-tag Protein (Catalog # 10951-PI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 89-98 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Reconstitute at 0.5 mg/mL in sterile, deionized water.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin G1/C1 Inhibitor
C1-inhibitor (C1-INH), also known as Serpin G1, is a 478 amino acid acute phase plasma glycoprotein member of the serpin superfamily of serine protease inhibitors. C1 inhibitor, like many other serpin family members, contains a highly conserved C-terminal serpin domain with a reactive center loop that protrudes to present a peptide for proteolytic attack (1). Binding disrupts the protease active site and traps it in a covalent complex with C1-inhibitor resulting in inactivation of the protease. C1 inhibitor also contains a highly glycosylated N- terminus and a unique C-terminal tail that has been suggested to serve as a barrier for transition to an inactive latent form (2). C1 inhibitor is synthesized and secreted primarily by hepatocytes but also by other cells such as monocytes, fibroblasts, and macrophages (1). C1 inhibitor is the sole physiological inhibitor of activated classical complement pathway serine proteases C1r and C1s (3). In addition, C1 inhibitor can inhibit multiple other serine proteases including plasma kallikrein and coagulation factor XIIa from the contact activation system, MASP-1 and MASP-2 proteases from the mannose binding lectin pathway, factor XIa and thrombin from the coagulation system, and plasmin and tissue plasminogen activator from the fibrinolytic system (3). Serpin G1 deficiency, whether caused by deletions, mutations, or inactivation, results in an autosomal dominantly inheritable potentially lethal disease, hereditary angioedema (HAE) (1). Some mutations in the SerpinG1 gene are also associated with age-related macular degeneration (4). Given the role C1-inhibitor plays as a potent anti-inflammatory agent, it has been developed for clinical use to treat HAE (1, 5) and considered for treatment of several other conditions including sepsis, acute myocardial infarction, brain injury, transplantation and COVID19 (6-11).
Karnaukhova, E. (2013) J. Hematol. Thromb. Dis. 1:3.
Beinrohr, L. et al. (2007) J. Biol. Chem. 282:21100.
Davis, A.E. III (2010) Thromb. Haemost. 104:886.
Ennis, S. et al. (2008) Lancet 372:1828.
Cicardi, M and A. Zanichelli (2010) Drugs Today. 46:867.
Caliezi, C. et al. (2000) Pharmacol. Rev. 52:91.
Thielmann, M. et al. (2006) Eur. J. Cardiothorac. Surg. 30:285.
Singer, M. and Jones, A.M. (2011) Crit. Care 15:203.
Berger, M. et al. (2016) Transplantation 100:1415.
Weiss, E. et al. (2020) Clin. Exp. Emerg. Med. 7:87.
Adesanya, T.M.A. et al. (2021) J. Clin. Immunol. 41:729.
Entrez Gene IDs
C1 Inhibitor, C1IN, C1INH, C1NH, HAE1, HAE2, SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 , Serpin G1
Citations for Recombinant Human Serpin G1 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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