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Recombinant Human Serpin F2/alpha 2-Antiplasmin Protein, CF

Catalog # 1470-PI | R&D Systems, Inc. a Bio-Techne Brand
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1470-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Serpin F2/alpha 2-Antiplasmin protein
Met28-Lys491, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met28

Predicted Molecular Mass

53 kDa

SDS-PAGE

67 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <2.0 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1470-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F2/alpha 2-Antiplasmin

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level. The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues (4). Human Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28-39) and a mature chain (residues 40-491). The presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (5).

References

  1. Tone, M. et al. (1987) J. Biochem. 102:1033.
  2. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  3. Potempa, J. et al. (1988) Science 241:699.
  4. Menoud, P.-A. et al. (1996) J. Clin. Invest. 97:2478.
  5. Sumi, Y. et al. (1989) J. Biochem. 106:703.

Alternate Names

alpha 2-Antiplasmin

Entrez Gene IDs

5345 (Human); 18816 (Mouse)

Gene Symbol

SERPINF2

UniProt

Additional Serpin F2/alpha 2-Antiplasmin Products

Product Documents for Recombinant Human Serpin F2/alpha 2-Antiplasmin Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Serpin F2/alpha 2-Antiplasmin Protein, CF

For research use only

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