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Recombinant Human Serpin A3 Protein, CF

Catalog # 1295-PI | R&D Systems, Inc. a Bio-Techne Brand
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1295-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Serpin A3/alpha 1-Antichymotrypsin protein
Asn26-Ala423, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn26

Predicted Molecular Mass

46 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <8 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1295-PI
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3/alpha 1-Antichymotrypsin

Serpin A3 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma (2). It is known to inhibit several serine proteases including chymotrypsin, cathepsin G, chymase, kallikrein 3/prostate specific antigen, and unidentified ectoenzymes that process pro-macrophage stimulating protein (1‑5). Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation (1‑6). Deficiency in Serpin A3 activity due to a point mutation (Leu55Pro) is associated with chronic obstructive pulmonary disease (7). Human Serpin A3 is synthesized as a 423 amino acid precursor (8, 9). The mature protein is secreted and has two forms that differ in their N‑termini (10). One of the forms starting at Asn26 was expressed and purified.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Kalsheker, N. et al. (2002) Biochem. Soc. Trans. 30:93.
  3. Duranto, J. et al. (1998) Biochemistry 37:11239.
  4. Hsieh, M.-C. and B.S. Cooperman (2002) Biochemistry 41:2990.
  5. Skeel, A. and E.J. Leonard (2001) J. Biol. Chem. 276:21932.
  6. Abraham, C.R. et al. (2000) Ann. N. Y. Acad. Sci. 920:245.
  7. Gooptu, B. et al. (2000) Proc. Natl. Acad. Sci. USA 97:67.
  8. Chandra, T. et al. (1983) Biochemistry 22:5055.
  9. Morii, M. and J. Travis (1983) J. Biol. Chem. 258:12749.
  10. Lindmark, B. et al. (1989) Biochim. Biophys. Acta 997:90.

Alternate Names

alpha 1-Antichymotrypsin

Entrez Gene IDs

12 (Human)

Gene Symbol

SERPINA3

UniProt

Product Documents for Recombinant Human Serpin A3 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Serpin A3 Protein, CF

For research use only

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