Product Specifications for Recombinant Human Serpin A3 Protein, CF
Mouse myeloma cell line, NS0-derived human Serpin A3/alpha 1-Antichymotrypsin protein Asn26-Ala423, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
60-65 kDa, reducing conditions
Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002). The IC50 value is <8 nM, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A3/alpha 1-Antichymotrypsin
Serpin A3 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma (2). It is known to inhibit several serine proteases including chymotrypsin, cathepsin G, chymase, kallikrein 3/prostate specific antigen, and unidentified ectoenzymes that process pro-macrophage stimulating protein (1‑5). Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation (1‑6). Deficiency in Serpin A3 activity due to a point mutation (Leu55Pro) is associated with chronic obstructive pulmonary disease (7). Human Serpin A3 is synthesized as a 423 amino acid precursor (8, 9). The mature protein is secreted and has two forms that differ in their N‑termini (10). One of the forms starting at Asn26 was expressed and purified.
Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Kalsheker, N. et al. (2002) Biochem. Soc. Trans. 30:93.
Duranto, J. et al. (1998) Biochemistry 37:11239.
Hsieh, M.-C. and B.S. Cooperman (2002) Biochemistry 41:2990.
Skeel, A. and E.J. Leonard (2001) J. Biol. Chem. 276:21932.
Abraham, C.R. et al. (2000) Ann. N. Y. Acad. Sci. 920:245.
Gooptu, B. et al. (2000) Proc. Natl. Acad. Sci. USA 97:67.
Chandra, T. et al. (1983) Biochemistry 22:5055.
Morii, M. and J. Travis (1983) J. Biol. Chem. 258:12749.
Lindmark, B. et al. (1989) Biochim. Biophys. Acta 997:90.
Entrez Gene IDs
AACTMGC88254, ACTGIG25, Cell growth-inhibiting gene 24/25 protein, Serpin A3, alpha 1-Antichymotrypsin, alpha-1-antichymotrypsin, antitrypsin), member 3, growth-inhibiting protein 24, growth-inhibiting protein 25, member 3, serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, serine (or cysteine) proteinase inhibitor, clade A, member 3, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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