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Recombinant Human Semaphorin 6C Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2219-S6

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2219-S6-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 6C protein
Human Semaphorin 6C
(Ala25 - Val601)
Accession # NP_112175
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

89.2 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.
The concentration of 5.0-10 μg/mL in the presence of 20 ng/mL of rhNT-3 (R&D Systems, Catalog # 267-N3) is sufficient to cause significant growth cone collapse.
Optimal concentrations should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2219-S6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6C

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 ‑ 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that are most like Class 1 invertebrate semaphorins in structure, and exhibit neuropilin-independent binding to specific plexin A receptors (1 ‑ 3). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53 - 64% within the Sema domain. Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Human Sema6C cDNA encodes 930 aa, including a 24 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 306 aa cytoplasmic portion. Alternate exon splicing creates a 922 aa short form (Sema6C.3) that is lacking aa 184 - 223 within the Sema domain, but contains 32 unique aa inserted after aa 586; postnatally, this form predominates in muscle (2, 3). A 962 aa form contains only the insert. The 930 aa “long form” predominates in brain, especially in areas of increased plasticity (4). Human Sema6C ECD shares 92%, 93%, 94%, 94%, 95% and 87% aa identity with corresponding mouse, rat, porcine, bovine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is down‑regulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

References

  1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
  3. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  4. Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
  5. Yoshida, Y. et al. (2006) Neuron 52:775.
  6. Svensson, A. et al. (2008) J. Mol. Hist. 39:5.

Alternate Names

Sema6C, Semaphorin Y

Entrez Gene IDs

10500 (Human); 20360 (Mouse)

Gene Symbol

SEMA6C

UniProt

Additional Semaphorin 6C Products

Product Documents for Recombinant Human Semaphorin 6C Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Semaphorin 6C Fc Chimera Protein, CF

For research use only

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