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Recombinant Human SCGF-alpha/CLEC11a Protein

Bio-Techne includes R&D Systems | Catalog # 1904-SC

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Carrier Free
1904-SC-025/CF

Catalog #
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With Carrier
1904-SC-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human SCGF/CLEC11a protein
Gly19-Phe323, with an N-terminal Met and a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34.7 kDa

SDS-PAGE

50 kDa, reducing conditions

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 0.12-0.6 μg/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1904-SC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1904-SC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCGF/CLEC11a

Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature human SCGF contains an N‑terminal Glu‑rich region with an RGD motif, a leucine zipper region, and a C‑terminal lectin‑like domain (1, 2). The RGD motif is absent in mouse and rat SCGF (1). Full length human SCGF (the alpha isoform) shares approximately 83% amino acid (aa) sequence identity with mouse and rat SCGF. A shorter SCGF beta isoform lacks 78 aa of the lectin domain (1‑3). This recombinant protein corresponds to the SCGF alpha isoform. SCGF is produced by various hematopoietic cell lines and by CD34+ and CD34‑CD33+ bone marrow cells as a 47 kDa sulfated and O‑glycosylated sialoprotein (2‑6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c‑Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU‑E) promoting activity in the absence of SCF/c‑kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL‑3, Flt‑3 Ligand, GM‑CSF, or G‑CSF to enhance the growth of granulocyte‑macrophage colonies (CFU‑GM) (4, 6). It also promotes (with Flt‑3 Ligand and VEGF) the maintenance and expansion of CD34+CD133+ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). Circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (-539C/T) is associated with resistance to malarial anemia (10, 11).

References

  1. Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.
  2. Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.
  3. Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.
  4. Hiraoka, A. et al. (1987) Cancer Res. 47:5025.
  5. Ito, C. et al. (2003) Bone Marrow Transplant. 32:391.
  6. Hiraoka, A. et al. (2001) Hematol. J. 2:307.
  7. Da Riva, L. et al. (2011) J. Transl. Med. 9:158.
  8. Gehling, U.M. et al. (2000) Blood 95:3106.
  9. Loges, S. et al. (2004) Stem Cells Dev. 13:229.
  10. Keller, C.C. et al. (2009) Infect. Immun. 77:3864.
  11. Ouma, C. et al. (2010) Infect. Immun. 78:453.

Long Name

Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A

Alternate Names

CLEC11a, CLECSF3, LSLCL

Entrez Gene IDs

6320 (Human); 20256 (Mouse); 29313 (Rat)

Gene Symbol

CLEC11A

UniProt

Additional SCGF/CLEC11a Products

Product Documents for Recombinant Human SCGF-alpha/CLEC11a Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human SCGF-alpha/CLEC11a Protein

For research use only

FAQs for Recombinant Human SCGF-alpha/CLEC11a Protein

Showing  1 - 1 of 1 FAQ Showing All
  • Q: This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

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