Recombinant Human SCF R/c-kit

R&D Systems, part of Bio-Techne | Catalog # 332-SR

Discontinued Product
332-SR has been discontinued. An alternative/replacement product is available: 332-SR/CF. View all CD117/c-kit products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P10721

Conjugate

Unconjugated

Applications

Bioactivity

View all CD117/c-kit Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CD117/c-kit protein
Gln26-Thr520
Accession # P10721

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26

Predicted Molecular Mass

55.7 kDa

SDS-PAGE

59-63 kDa, reducing conditions

Activity

Measured by its ability to inhibit SCF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 2-6 µg/mL in the presence of 8 ng/mL of recombinant human SCF.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 332-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 332-SR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem Cell Factor Receptor (SCF R), also known as c-Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v-Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1-4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature human SCF R consists of a 499 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin-like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, human SCF R shares 73% and 76% aa sequence identity with mouse and rat SCF R, respectively. Alternative splicing of human SCF R generates a potentially secreted isoform that corresponds to the first four Ig-like domains. SCF is expressed as transmembrane and soluble noncovalent homodimers (8). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (8). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (9). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (11, 12). SCF is a primary growth and activation factor for mast cells and eosinophils (13). SCF R expression on mast cells enables them to infiltrate SCF-secreting tumors where they promote tumor growth and induce local immune suppression (14). SCF R is up‑regulated on dendritic cells by Th2- or Th17-biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (15). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (16, 17).

References

  1. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  2. Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
  3. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  4. Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
  5. Pittoni, P. et al. (2011) Oncogene 30:757.
  6. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
  7. Yarden, Y. et al. (1987) EMBO J. 6:3341.
  8. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  9. Miyazawa, K. et al. (1995) Blood 85:641.
  10. Martin, F.H. et al. (1990) Cell 63:203.
  11. Turner, A.M. et al. (1995) Blood 85:2052.
  12. Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
  13. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  14. Huang, B. et al. (2008) Blood 112:1269.
  15. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  16. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  17. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P10721

Additional CD117/c-kit Products

Product Documents for Recombinant Human SCF R/c-kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 332-SR (with carrier)
Download SDS 332-SR/CF (carrier free)

Product Specific Notices for Recombinant Human SCF R/c-kit

For research use only

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