Breadcrumb
- Home
- Products
- Recombinant Human SCF GMP Protein, CF (255B-GMP)


Order Information
Key Product Details
Source: | E. coli |
Accession #: | P21583.1 |
Applications: | Bioactivity |
Complete Your Experiment
Complete Your Research
Product Specifications
Source |
E. coli-derived human SCF/c-kit Ligand protein Glu26-Ala189, with an N-terminal Met. Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines. |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. The result from mass spectrometry confirms the major peak corresponds to SCF/c-kit ligand. See the CofA for additional information |
Endotoxin level | <0.10 EU per 1 μg of the protein by the LAL method. |
N-terminal sequence Analysis | Met-Glu26-Gly-Ile-(Cys)-Arg-Asn-Arg-Val-Thr |
Predicted Molecular Mass | 19 kDa |
SDS-PAGE | 19 kDa, reducing conditions |
Activity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.The specific activity of recombinant human SCF is >1.0 x 106 units/mg, which is calibrated against the human SCF reference standard (NIBSC code: 91/682). |
![]() |
Recombinant Human SCF GMP Protein Bioactivity GMP-grade Recombinant Human SCF (Catalog # 255B-GMP) stimulates cell proliferation of the TF‑1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL. Four independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP SCF. |
![]() |
Recombinant Human SCF GMP Protein SDS-PAGE 2 μg/lane of GMP-grade Recombinant Human SCF (Catalog # 255B-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing R band at 18 kDa and NR band at 16 kDa, respectively. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SCF/c-kit Ligand
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).
Item | Value |
---|---|
References |
|
Long Name | Stem Cell Factor |
Entrez Gene IDs | 4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline) |
Alternate Names | DCUA, DFNA69, DKFZp686F2250, FPH2, FPHH, KIT ligand, KITLG, KL-1, Kitl, MGF, MGFSHEP7, Mast cell growth factor, SCF, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, c-kit Ligand, familial progressive hyperpigmentation 2, steel factor |
Citations
Currently there are no citations for Recombinant Human SCF GMP Protein, CF.