Recombinant Human SCF, Animal-Free Protein

Name: Recombinant Human SCF, Animal-Free Protein
Brand: R&D Systems
SKU: AFL255

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AFL255

Bigger Scale, More Savings. Try our NEW version of Animal Free Human Recombinant SCF (BT-SCF-AFL). Combining R&D Systems quality with scalability that allows for lower price points and a solid supply chain.

Also available, GMP SCF Protein, Catalog # 255B-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

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Key Product Details

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Source:	E. coli
Accession #:	P21583.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human SCF, Animal-Free Protein

Product Specifications for Recombinant Human SCF, Animal-Free Protein

Source	E. coli-derived human SCF/c-kit Ligand protein Glu26-Ala189, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	19 kDa
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1‑5 ng/mL. The specific activity of recombinant human SCF is >1.0 x 10⁶ units/mg, which is calibrated against the human SCF reference standard (NIBSC code: 91/682).

Scientific Data Examples for Recombinant Human SCF, Animal-Free Protein

	Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human SCF Equivalent bioactivity of GMP (255B-GMP), Animal-Free (Catalog # AFL255), and RUO (255-SC/CF) grades of Recombinant Human SCF as measured in cell proliferation assay (orange, green, red, respectively).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL255

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

Item	Value
References	Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. Kapur, R. et al. (2002) Blood 100:1287. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. Martin, F.H. et al. (1990) Cell 63:203. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Long Name	Stem Cell Factor
Entrez Gene IDs	4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names	DCUA, DFNA69, DKFZp686F2250, FPH2, FPHH, KIT ligand, KITLG, KL-1, Kitl, MGF, MGFSHEP7, Mast cell growth factor, SCF, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, c-kit Ligand, familial progressive hyperpigmentation 2, steel factor

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

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Recombinant Human SCF, Animal-Free Protein

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Product Specifications for Recombinant Human SCF, Animal-Free Protein

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Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: SCF/c-kit Ligand

Manufacturing Specifications

Citations for Recombinant Human SCF, Animal-Free Protein

Product Documents for Recombinant Human SCF, Animal-Free Protein

Product Datasheets

COA

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SDS

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