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Recombinant Human S100A8/S100A9 Heterodimer Protein, CF Best Seller
Catalog # 8226-S8 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
E. coli
Structure / Form
Noncovalently-linked heterodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
E. coli-derived human S100A8/S100A9 Heterodimer protein
Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1 (S100A8) & Thr2 (S100A9)
Predicted Molecular Mass
11 kDa (S100A8) & 13 kDa (S100A9)
SDS-PAGE
9 kDa (S100A8) & 13 kDa (S100A9), reducing conditions
Activity
Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172.
The ED50 for this effect is 1.5-6 μg/mL.
The ED50 for this effect is 1.5-6 μg/mL.
Scientific Data Images for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF
Recombinant Human S100A8/S100A9 Heterodimer Protein Bioactivity
Recombinant Human S100A8/S100A9 Heterodimer (Catalog # 8226-S8) induces IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is 1.5-6 μg/mL.Formulation, Preparation and Storage
8226-S8
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: S100A8/S100A9 Heterodimer
EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).
References
- Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
- Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
- Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
- Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
- Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
- Odink, K. et al. (1987) Nature 330:80.
- Dorin, J.R. et al. (1987) Nature 326:614.
- Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
- Ryckman, C. et al. (2003) J. Immunol. 170:3233.
- Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
- Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
- Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
Long Name
S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Alternate Names
Calprotectin
Entrez Gene IDs
6279 (Human)
Gene Symbol
S100A8
Product Documents for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF
Product Specific Notices for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF
For research use only
Citations for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF (9)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF include:
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Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZ Li et al. (2022-04-19), ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation Nature Communications, 2022-013(1):2011.
PMID: 35440136 -
Species: Human
Sample Types: Plasma
Applications: Flow CytometryLGN de Almeida et al. (2022-04-07), Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia Cells, 2022-011(8).
PMID: 35455936 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayN Maehara et al. (2021-09-14), AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-036(11):109693.
PMID: 34525359 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDG Bisson et al. (2020-08-27), Toll-like receptor involvement in adolescent scoliotic facet joint degeneration J. Cell. Mol. Med., 2020-00(0).
PMID: 32853438 -
Species: Human
Applications: Cell CultureV Rolfes et al. (2020-05-12), Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020-031(6):107615.
PMID: 32402278 -
Species: Human
Sample Types: Plasma
Applications: BioassayC Dubois et al. (2019-10-30), High plasma level of S100A8/S100A9 and S100A12 at admission indicates a higher risk of death in septic shock patients Sci Rep, 2019-19(1):15660.
PMID: 31666644 -
Species: Rat
Sample Types: Whole Cells
Applications: BioassayJ Sun et al. (2018-06-09), Necrotizing enterocolitis is associated with acute brain responses in preterm pigs J Neuroinflammation, 2018-015(1):180.
PMID: 29885660 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayA Basu et al. (2018-04-18), A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing Sci Rep, 2018-08(1):6205.
PMID: 29670130
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Customer Reviews for Recombinant Human S100A8/S100A9 Heterodimer Protein, CF (1)
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