Recombinant Human ROBO2 Fc Chimera Protein, CF

Name: Recombinant Human ROBO2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 3147-RB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3147-RB

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_001276969
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human ROBO2 Fc Chimera Protein, CF

Product Specifications for Recombinant Human ROBO2 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human ROBO2 protein

Human ROBO2 (Ser22-Pro863) Accession # NP_001276969	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser22

Predicted Molecular Mass

120 kDa (monomer)

SDS-PAGE

146-160 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Slit-3 immobilized on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Human ROBO2 Fc Chimera with an apparent K_d <10 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3147-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31-504) and three fibronectin (FN) type III domains (aa 522-826). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186-1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22 - 285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD is 98% aa identical to the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1‑ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).

Item	Value
References	Rajagopalan, S. et al. (2000) Neuron 28:767. Guthrie, S. (2004) Curr. Biol. 14:R632. Guthrie, S. (2001) Curr. Biol. 11:R300. Seeger, M. et al. (1993) Neuron 10:409. Kidd, T. et al. (1998) Cell 92:205. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281. Bashaw, G.J. et al. (2000) Cell 101:703. Jen, J.C. et al. (2004) Science 304:1509. GenBank Accession # Q9KCK4. Isogai, T. et al. (2002) GenBank Accession # AK074780. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41. Long, H. et al. (2004) Neuron 42:213. Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534. Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.
Long Name	Roundabout Homolog 2
Entrez Gene IDs	6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names	Drosophila) homolog 2, ROBO2, SAX3, roundabout, axon guidance receptor, homolog 2 (Drosophila)

Citations for Recombinant Human ROBO2 Fc Chimera Protein, CF (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ROBO2 Fc Chimera Protein, CF include:

Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

AN Gonçalves et al. (2020), ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia Respir Res, 21(1):302.
PMID: 33208157
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Greaves E et al. (2014), Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3. Endocrinology, 155(10):4015-26.
PMID: 25051436
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Ricano-Cornejo I et al. (2011), Slit-Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain. J. Neurosci. Res., 89(10):1531-41.
PMID: 21688288

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Recombinant Human ROBO2 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Human ROBO2 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased