Product Specifications for Recombinant Human Resistin Protein, CF
E. coli-derived human Resistin protein Ser17-Pro108
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
10 kDa, reducing conditions.
Bioassay data are not available.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Reconstitute at 50 μg/mL in 2 mM acetic acid, pH 3.0.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Resistin (resistance-to-insulin), also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis (1, 2, 3, 4). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. The mature region shows an N-terminal alpha -helical tail (aa 23 ‑ 44) and a C-terminal beta ‑sheet globular head (aa 47 ‑ 108) (5 ‑ 7). The Resistin molecule circulates as either a noncovalent trimer (minor form), or a disulfide-linked homohexamer (major form). Noncovalent trimers are generated when the alpha -helical segments self-associate to form a three-stranded coiled-coil structure. Covalent hexamers subsequently appear when the free Cys at position # 26 is engaged by adjacent trimers. It is hypothesized that the hexamer is the inactive form of the molecule, and bioactivity is achieved at the target site by disulfide bond reduction (5). Although Resistin family molecules can noncovalently interact to form heterotrimers in vitro, there is no evidence to suggest this occurs in vivo with Resistin (8, 9). Mature human Resistin shares 56% and 54% aa identity with mouse and rat Resistin, respectively. Rat Resistin possesses an alternate start site at Met48; this Met is not found in the mouse molecule, however (10). Rodent Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium (6, 11, 12). Although the function of Resistin is unclear, it would seem to block insulin-stimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes (6, 13, 14). As such, it has been proposed to participate in diet‑induced insulin-sensitivity. Diets high in fat promote an increase in overall adipocyte size. Hypertrophic adipocytes are known to secrete TNF-alpha which acts locally to block ACRP30 production. Since ACRP30 is an insulin-sensitizer, a drop in ACRP30 availability leads to insulin-insensitivity, which drives increased insulin production (a compensatory mechanism). High insulin induces Resistin secretion which now antagonizes insulin action, prompting more insulin production and more Resistin secretion (15).
Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
King, I.A. et al. (1993) Genomics 18:185.
Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.
Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
Chidgey, M. et al. (2001) J. Cell Biol. 155:821.
Khan, K. et al. (2006) Br. J. Cancer 95:1367.
Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.
Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.
Entrez Gene IDs
56729 (Human); 57264 (Mouse)
ADSF, ADSFMGC126609, Adipose tissue-specific secretory factor, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, FIZZ3MGC126603, HXCP1, RETN, RETN1, RSTNXCP1, Resistin, found in inflammatory zone 3
Product Specific Notices for Recombinant Human Resistin Protein, CF
The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development.
This product is manufactured under a license to U.S. Patent number 7,470,522. Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.
MAB13591 was used as the capture antibody in a sandwich ELISA along with AF1359 as the detection. We also tested BAF1359 as the detection since in comes biotinylated. The standard for the ELISA was 1359-RN. The assay worked excellently! It was sensitive and specific and detected all human samples we tested at a 1:4 dilution.
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