Recombinant Human Relaxin-2 (B-33/A-24) Protein

Name: Recombinant Human Relaxin-2 (B-33/A-24) Protein
Brand: R&D Systems
SKU: 3596-RN
Price: 394 USD

Catalog # 3596-RN | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

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Key Product Details

Source:	E. coli
Accession #:	P04090
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human Relaxin-2 (B-33/A-24) Protein

Source	E. coli-derived human Relaxin-2 protein Val23-Arg55 (B chain), with an N-terminal Met & Gln162-Cys185 (A chain)
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met (B chain) & Gln162 (A chain)
Predicted Molecular Mass	2.7 kDa (A chain), 3.9 kDa (B chain)
Activity	Measured by its ability to induce cAMP accumulation in THP‑1 human acute monocytic leukemia cells. Parsell, D.A. et al. (1996) J. Biol. Chem. 271:27936. The ED₅₀ for this effect is 0.5‑2.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3596-RN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3596-RN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Relaxin-2

Human Relaxin-2, also called H2 Relaxin, is a 6 kDa, 53 amino acid (aa) nonglycosylated, heterodimeric polypeptide that plays an important role in female reproduction (1, 2). Relaxin belongs to a structurally related insulin/relaxin superfamily that currently contains 10 members in human (2). To date, three human relaxin genes have been identified (1, 2). Among these, Relaxin-2 is best studied and the only known Relaxin to circulate in the blood (2, 3). As with other insulin/relaxin superfamily members, human Relaxin-2 is synthesized as a preprohormone (4). It is 18 kDa in size and 185 aa in length. It contains a 24 aa signal sequence, a 3.3 kDa, 31 aa B domain, a 106 aa C (or connecting) domain, and a C-terminal, 2.7 kDa, 24 aa A domain (2, 4, 5). Upon removal of the signal peptide, two intrachain disulfide bonds are created between the B and A chains. This is followed by prohormone convertase removal of the intervening C chain, creating a disulfide-linked heterodimer. Initially, the B chain is 31 aa in length and terminates with a Lys‑Arg dipeptide. This is subsequently cleaved by a carboxypeptidase to generate a 29 aa mature chain (5). The mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively. Human Relaxin-2 is 35% and 75% aa identical to human Relaxin-3 and 1, respectively. An alternate splice form for human Relaxin-2 has been reported (6). It is identical to the standard form through the first 70 aa of the preproprecursor. At this point, a 47 aa substitution occurs that appears to be absent in typical cleavage motifs. Relaxin confers its activity by binding to leucine-rich guanine nucleotide-binding (G-protein) coupled receptors, LGR7 and LGR8 (2, 7). Relaxin is best known as a hormone of parturition that promotes growth and softening of the cervix, and development of the mammary gland (2, 3). It also has a marked impact on the uterus. In particular, it promotes angiogenesis, inhibits MMP production and activity, and down‑regulates estrogen receptor-alpha expression (8).

Item	Value
References	Hayes, E.S. (2004) Reprod Biol Endocrinol. 2:36. Sherwood, O.D. (2004) Endocr. Rev. 25:205. Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14. Hudson, P. et al. (1984) EMBO J. 3:2333. Marriott, D. et al. (1992) Mol. Endocrinol. 6:1441. Gunnersen, J.M. et al. (1996) Mol. Cell. Endocrinol. 118:85. Hsu, S.Y. et al. (2002) Science. 295:671. Goldsmith, L.T. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4685.
Entrez Gene IDs	6019 (Human)
Alternate Names	H2, RLN2, RLXH2, Relaxin-2, Relaxin2, bA12D24.1.1, bA12D24.1.2, relaxin 2

Citations for Recombinant Human Relaxin-2 (B-33/A-24) Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Relaxin-2 (B-33/A-24) Protein include:

Species: Hamster, Human
Sample Types: Whole Cells
Applications: Bioassay

J Sun et al. (2019-12-10), Human Relaxin-2 Fusion Protein Treatment Prevents and Reverses Isoproterenol-Induced Hypertrophy and Fibrosis in Mouse Heart J Am Heart Assoc, 2019-18(24):e013465.
PMID: 31818212
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

KC Lee et al. (2019-03-27), Human relaxin-2 attenuates hepatic steatosis and fibrosis in mice with non-alcoholic fatty liver disease Lab. Invest., 2019-00(0).
PMID: 30918325
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

H Kamimoto et al. (2019-03-14), Relaxin 2 carried by magnetically directed liposomes accelerates rat midpalatal suture expansion and subsequent new bone formation Bone Rep, 2019-010(0):100202.
PMID: 30937342
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Krueger C et al. (2010-11-30), Identification of retinoic acid in a high content screen for agents that overcome the anti-myogenic effect of TGF-beta-1. PLoS ONE, 2010-15(11):e15511.
PMID: 21152098

Recombinant Human Relaxin-2 (B-33/A-24) Protein

Catalog # 3596-RN | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Relaxin-2 (B-33/A-24) Protein

Formulation, Preparation and Storage

Carrier Free

3596-RN

Carrier

3596-RN/CF

Carrier Free

Reconstitution Calculator

Background: Relaxin-2

Citations for Recombinant Human Relaxin-2 (B-33/A-24) Protein (4)

Product Documents for Recombinant Human Relaxin-2 (B-33/A-24) Protein

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