Product Specifications for Recombinant Human Reg1B Protein, CF
E. coli-derived human Reg1B protein Gln23-Asn166, with an N-terminal Met
>95%, by SDS-PAGE with silver staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
16 kDa, reducing conditions
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL.
Scientific Data Examples for Recombinant Human Reg1B Protein, CF
Recombinant Human Reg1B Protein Bioactivity
Recombinant Human Reg1B (Catalog # 2090-RG) stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL.
Recombinant Human Reg1B Protein SDS-PAGE
1 μg/lane of Recombinant Human Reg1B (Catalog # 2090-RG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 17.2 and 16.7 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).
Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
Livesey F.J. et al. (1990) Nature. 390:614.
Regenerating Islet-derived 1B
Entrez Gene IDs
Lithostathine 1 beta, PSPS2, PSPS2PSP-2, Pancreatic stone protein 2, REGH, REGLREG-1-beta, Reg1B, Regenerating islet-derived protein 1-beta, Regenerating protein I beta, lithostathine-1-beta, pancreatic threadprotein), regenerating islet-derived 1 beta, secretory pancreatic stone protein 2
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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