Recombinant Human PTPR sigma Protein, CF

Name: Recombinant Human PTPR sigma Protein, CF
Brand: R&D Systems
SKU: 3430-PR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3430-PR

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_570924
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PTPR sigma Protein, CF

Complete Your Research

Product Specifications for Recombinant Human PTPR sigma Protein, CF

Source	Mouse myeloma cell line, NS0-derived human PTP sigma/PTPRS protein Glu30-Gly1260, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu30 & Ser1173
Predicted Molecular Mass	135.8 kDa
SDS-PAGE	120-145 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human PTPR sigma /PTPRS is coated at 2 μg/mL, Recombinant Human TrkC Fc Chimera (Catalog # 373‑TC) binds with an apparent K_d <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3430-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PTP sigma/PTPRS

Phosphatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma, is a 200 kDa type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPR sigma cDNA encodes 1948 amino acids (aa) including a 29 aa signal sequence, a 1253 aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPR sigma, releasing the 140-160 kDa ECD (3). The human PTPR sigma ECD shares 93% aa sequence identity with the nearest isoform of mouse and rat PTPR sigma, 96% with bovine PTPR sigma, and 97% with porcine PTPR sigma. Activity of PTPR sigma can be inhibited by bisphosphonates (2). PTPR sigma is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPR sigma affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPR sigma show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPR sigma (14). Since PTPR sigma inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).

Item	Value
References	Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686. Endo, N. et al. (1996) J. Bone Miner. Res. 11:535. Aicher, B. et al. (1997) J. Cell Biol. 138:681. Shen, Y. et al. (2009) Science 326:592. Duan, Y. and R.J. Giger (2010) Sci. Signal. 3:pe6. Siu, R. et al. (2007) Mol. Cell. Biol. 27:208. Vijayvargia, R. et al. (2004) Biochem. Biophys. Res. Commun. 325:344. Uetani, N. et al. (2009) J. Clin. Invest. 119:924. Muise, A.M. et al. (2007) Curr. Biol. 17:1212. Elchebly, M. et al. (1999) Nat. Genet. 21:330. Batt, J. et al. (2002) Mol. Endocrinol. 16:155. Chagnon, M.J. et al. (2006) Can. J. Physiol. Pharmacol. 84:755. McLean, J. et al. (2002) J. Neurosci. 22:5481. Morris, L.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:19024.
Long Name	Protein Tyrosine Phosphatase Receptor-type Sigma
Entrez Gene IDs	5802 (Human); 19280 (Mouse)
Alternate Names	EC 3.1.3.48, PTP sigma, PTPR sigma, PTPRS, PTPSIGMA, R-PTP-S, R-PTP-sigma, Receptor-type tyrosine-protein phosphatase sigma, protein tyrosine phosphatase PTPsigma, protein tyrosine phosphatase, receptor type, S, protein tyrosine phosphatase, receptor type, sigma, receptor-type tyrosine-protein phosphatase S

Citations for Recombinant Human PTPR sigma Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human PTPR sigma Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human PTPR sigma Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PTP sigma/PTPRS

Citations for Recombinant Human PTPR sigma Protein, CF

Product Documents for Recombinant Human PTPR sigma Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected