Product Specifications for Recombinant Human PTPR sigma Protein, CF
Mouse myeloma cell line, NS0-derived human PTP sigma/PTPRS protein Glu30-Gly1260, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Glu30 & Ser1173
Predicted Molecular Mass
120-145 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human PTPR sigma /PTPRS is coated at 2 μg/mL, Recombinant Human TrkC Fc Chimera (Catalog # 373‑TC) binds with an apparent Kd <1 nM.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP sigma/PTPRS
Phosphatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma, is a 200 kDa type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPR sigma cDNA encodes 1948 amino acids (aa) including a 29 aa signal sequence, a 1253 aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPR sigma, releasing the 140-160 kDa ECD (3). The human PTPR sigma ECD shares 93% aa sequence identity with the nearest isoform of mouse and rat PTPR sigma, 96% with bovine PTPR sigma, and 97% with porcine PTPR sigma. Activity of PTPR sigma can be inhibited by bisphosphonates (2). PTPR sigma is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPR sigma affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPR sigma show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPR sigma (14). Since PTPR sigma inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).
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Aicher, B. et al. (1997) J. Cell Biol. 138:681.
Shen, Y. et al. (2009) Science 326:592.
Duan, Y. and R.J. Giger (2010) Sci. Signal. 3:pe6.
Siu, R. et al. (2007) Mol. Cell. Biol. 27:208.
Vijayvargia, R. et al. (2004) Biochem. Biophys. Res. Commun. 325:344.
Uetani, N. et al. (2009) J. Clin. Invest. 119:924.
Muise, A.M. et al. (2007) Curr. Biol. 17:1212.
Elchebly, M. et al. (1999) Nat. Genet. 21:330.
Batt, J. et al. (2002) Mol. Endocrinol. 16:155.
Chagnon, M.J. et al. (2006) Can. J. Physiol. Pharmacol. 84:755.
McLean, J. et al. (2002) J. Neurosci. 22:5481.
Morris, L.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:19024.
Protein Tyrosine Phosphatase Receptor-type Sigma
Entrez Gene IDs
5802 (Human); 19280 (Mouse)
EC 22.214.171.124, PTP sigma, PTPR sigma, PTPRS, PTPSIGMA, R-PTP-S, R-PTP-sigma, Receptor-type tyrosine-protein phosphatase sigma, protein tyrosine phosphatase PTPsigma, protein tyrosine phosphatase, receptor type, S, protein tyrosine phosphatase, receptor type, sigma, receptor-type tyrosine-protein phosphatase S
Citations for Recombinant Human PTPR sigma Protein, CF
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Product Documents for Recombinant Human PTPR sigma Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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