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Recombinant Human PTP1B Protein, CF
Catalog # 1366-PT | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
E. coli-derived human PTP1B protein
Glu2-Asn321, with an N-terminal Met and 6-His tag
Glu2-Asn321, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
38 kDa
SDS-PAGE
38 kDa, reducing conditions
Activity
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006).
The specific activity is >15 μmol/min/mg, as measured under the described conditions.
The specific activity is >15 μmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
1366-PT
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and DTT. |
| Reconstitution | Reconstitute to 100 μg/mL in sterile 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, and 500 μg/mL bovine serum albumin, pH 7.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PTP1B
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta-catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.
References
- Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
- Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
- Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
- Balsamo, et al. (1996) J. Cell Biol. 134:801.
- Elchebly, et al. (1999) Science 283:1544.
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PTPN1
Gene Symbol
PTPN1
UniProt
Product Documents for Recombinant Human PTP1B Protein, CF
Product Specific Notices for Recombinant Human PTP1B Protein, CF
For research use only
Citations for Recombinant Human PTP1B Protein, CF (2)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PTP1B Protein, CF include:
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Species: Human
Sample Types: Protein
Applications: BioassayQiang et al. (2014-04-01), Identification of a small molecular insulin receptor agonist with potent antidiabetes activity. Diabetes, 2014-063(4):1394-409.
PMID: 24651808 -
Species: Mouse
Sample Types: N/A,Whole Cells
Applications: Western Blot,BioassayBorza CM et al. (2010-10-12), Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. J. Biol. Chem., 2010-1285(51):40114-24.
PMID: 20940300
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