Recombinant Human PTP1B Protein, CF
Catalog # 1366-PT | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Glu2-Asn321, with an N-terminal Met and 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >15 μmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
1366-PT
Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and DTT. |
Reconstitution | Reconstitute to 100 μg/mL in sterile 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, and 500 μg/mL bovine serum albumin, pH 7.5. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: PTP1B
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta-catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.
References
- Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
- Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
- Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
- Balsamo, et al. (1996) J. Cell Biol. 134:801.
- Elchebly, et al. (1999) Science 283:1544.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human PTP1B Protein, CF
Product Specific Notices for Recombinant Human PTP1B Protein, CF
For research use only
Citations for Recombinant Human PTP1B Protein, CF (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PTP1B Protein, CF include:
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Species: Human
Sample Types: Protein
Applications: BioassayQiang et al. (2014-04-01), Identification of a small molecular insulin receptor agonist with potent antidiabetes activity. Diabetes, 2014-063(4):1394-409.
PMID: 24651808 -
Species: Mouse
Sample Types: N/A,Whole Cells
Applications: Western Blot,BioassayBorza CM et al. (2010-10-12), Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. J. Biol. Chem., 2010-1285(51):40114-24.
PMID: 20940300
There are no citations that match your criteria.
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