Product Specifications for Recombinant Human PTP1B Protein, CF
E. coli-derived human PTP1B protein Glu2-Asn321, with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
38 kDa, reducing conditions
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006). The specific activity is >15 μmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and DTT.
Reconstitute to 100 μg/mL in sterile 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, and 500 μg/mL bovine serum albumin, pH 7.5.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.
Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
Balsamo, et al. (1996) J. Cell Biol. 134:801.
Elchebly, et al. (1999) Science 283:1544.
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs
5770 (Human); 19246 (Mouse)
EC 18.104.22.168, PTP-1B, PTP1B, PTP1Bprotein tyrosine phosphatase, placental, PTPN1, Protein-tyrosine phosphatase 1B, protein tyrosine phosphatase 1B, protein tyrosine phosphatase, non-receptor type 1, tyrosine-protein phosphatase non-receptor type 1
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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