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Recombinant Human PTP1B Protein, CF

Catalog # 1366-PT | R&D Systems, Inc. a Bio-Techne Brand
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1366-PT-050

Key Product Details

Accession #

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human PTP1B protein
Glu2-Asn321, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006).
The specific activity is >15 μmol/min/mg, as measured under the described conditions.

Formulation, Preparation and Storage

1366-PT
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and DTT.
Reconstitution Reconstitute to 100 μg/mL in sterile 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, and 500 μg/mL bovine serum albumin, pH 7.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta-catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

References

  1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
  2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
  3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
  4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
  5. Elchebly, et al. (1999) Science 283:1544.

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Entrez Gene IDs

5770 (Human); 19246 (Mouse)

Gene Symbol

PTPN1

UniProt

Product Documents for Recombinant Human PTP1B Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PTP1B Protein, CF

For research use only

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  • Recombinant Human PTP1B Protein, CF
    Name: Sivanesan Dakshanamurthy
    Application: SPR assay
    Verified Customer | Posted 01/30/2019

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