Product Specifications for Recombinant Human PRPS1 His-tag Protein, CF
E. coli-derived human PRPS1 protein Pro2-Leu318 with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
Measured by its ability to convert D-ribose 5-phosphate to 5-phospho-alpha-D-ribose.
The specific activity is >25 pmol/min/μg, as measured under the described conditions.
Scientific Data Examples for Recombinant Human PRPS1 His-tag Protein, CF
Recombinant Human PRPS1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human PRPS1 His-tag Protein (Catalog # 11075-PS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and TCEP.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Ribose-phosphate pyrophosphokinase 1 (PRPS1), also known as phosphoribosyl pyrophosphate synthase I, is a highly conserved, ubiquitously expressed enzyme from the ribose-phosphate pyrophosphokinase family that catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) from adenosine triphosphate (ATP) and ribose-5-phosphate (R5P). It is a crucial enzyme in the de novo synthesis and salvage of purines and biosynthesis of pyrimidine and pyrimidine nucleotides (1, 2). PRPS1 is activated by inorganic phosphate and magnesium and can be allosterically inhibited by ADP and purines (2, 3). Human PRPS1 is a 318 amino acid monomer that forms an active hexamer consisting of three homodimers arranged in a propellar-like shape (4). Each homodimer has an active site that binds both ATP and R5P as well as an allosteric inhibitor site. It has been well-established that many different mutations in the PRPS1 gene can lead to disease. Mutations can result in a gain of function with increased expression that leads to excess purine production present in PRS-I superactivity characterized by gout, hearing loss, hypotonia, and ataxia (2, 5). Alternatively, mutations can result in loss of function with decreased expression present in nonsyndromic sensorineural deafness (DFN-2), Charcot-Marie-Tooth disease-5 (CMTX5), and Arts syndrome characterized by sensorineural hearing loss, optic atrophy, ataxia, neuropathy motor development delay and intellectual disability (2,5,6). Mis-regulation of expression and mutation of PRPS1 expression has also been shown to promote proliferation in cancers including neuroblastoma (7), squamous cell carcinoma (8), and acute lymphoblastic leukaemia (9). Additionally, PRPS1 has been shown to be regulated through its phosphorylation state to play a role in DNA repair in the innate immune response (10) and promote tumorigenesis (11).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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