Source	Chinese Hamster Ovary cell line, CHO-derived human Plexin B1 protein Leu20-Gln535, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu20
Predicted Molecular Mass	56 kDa
SDS-PAGE	60-68 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B1 His-tag is coated at 1.00 µg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4) binds with an ED₅₀ of 80.0-480 ng/mL.

Recombinant Human Plexin B1 (Sema domain) His Protein, CF Scientific Data Examples

	Recombinant Human Plexin B1 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human Plexin B1 His-tag (Catalog # 10803-PB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.
	Recombinant Human Plexin B1 (Sema domain) His-tag Protein Binding Activity When Recombinant Human Plexin B1 (Sema domain) His-tag (Catalog # 10803-PB) is coated at 1.00 µg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera [[catalogNumber:(7470-S4)]] binds with an ED₅₀ of 80.0-480 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10803-PB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin B1

Plexin B1, also known as SEP, is a type I transmembrane glycoprotein that belongs to the Plexin family of semaphorin receptors. In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (1). Plexins share a common structural architecture: A large extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and a conserved ~600 amino acid (aa) cytoplasmic region (2, 3). Human Plexin B1 contains one Semaphorin domain, two plexin-semaphorin-integrin (PSI) domains, and three immunoglobin-plexin-transcription factor (IPT) repeats in the ECD. The mature ECD of human Plexin B1 shares 82% aa sequence identity with the ECD of mouse Plexin B1. Like semaphorins, plexins can be soluble or membrane bound and Plexin B1 can be cleaved into an ~200 kDa, soluble alpha -subunit and an ~100 kDa membrane bound beta -subunit (2). Plexin B1 is a receptor for Semaphorin 4D/CD100 and it can form a receptor complex with Neuropilins, MET, and EGF R2 (4). Plexin B1 is expressed in chick retinal ganglion neurons and hippocampal neurons with a peak during the myelination period (5). Plexin B1 also plays a role in GABAergic development, changes of actin cytoskeleton, axon guidance, cell migration, and invasive growth (6, 7).

Item	Value
References	Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789 Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094. Negishi, M. et al. Cell. Mol. Life Sci. (2005) 62:1363. Franco, M. and Tamagnone, L. (2008) EMBO Rep. 9:865. Moreau-Fauvarque, C. et al. J. Neurrosci (2003) 23:9229. Swiercz, J. et al. J Cell Biol (2004) 165(6):869. Giordano, S. et al. Nat Cell Biol. (2002) 4(9):720.
Entrez Gene IDs	5364 (Human); 235611 (Mouse); 316009 (Rat)
Alternate Names	PLXN5, PLXNB1, Plexin B1, SEP, Transmembrane Protein SEP

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Recombinant Human Plexin B1 (Sema domain) His Protein, CF

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