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Recombinant Human Plexin B1 (Sema domain) His Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10803-PB

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10803-PB-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Plexin B1 protein
Leu20-Gln535, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

56 kDa

SDS-PAGE

60-68 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Plexin B1 His-tag is coated at 1.00 µg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4) binds with an ED50 of 80.0-480 ng/mL.

Scientific Data Images for Recombinant Human Plexin B1 (Sema domain) His Protein, CF

Recombinant Human Plexin B1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Plexin B1 His-tag (Catalog # 10803-PB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.

Recombinant Human Plexin B1 (Sema domain) His-tag Protein Binding Activity

When Recombinant Human Plexin B1 (Sema domain) His-tag (Catalog # 10803-PB) is coated at 1.00 µg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (7470-S4) binds with an ED50 of 80.0-480 ng/mL.

Formulation, Preparation and Storage

10803-PB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B1

Plexin B1, also known as SEP, is a type I transmembrane glycoprotein that belongs to the Plexin family of semaphorin receptors. In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (1). Plexins share a common structural architecture: A large extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and a conserved ~600 amino acid (aa) cytoplasmic region (2, 3). Human Plexin B1 contains one Semaphorin domain, two plexin-semaphorin-integrin (PSI) domains, and three immunoglobin-plexin-transcription factor (IPT) repeats in the ECD. The mature ECD of human Plexin B1 shares 82% aa sequence identity with the ECD of mouse Plexin B1. Like semaphorins, plexins can be soluble or membrane bound and Plexin B1 can be cleaved into an ~200 kDa, soluble alpha-subunit and an ~100 kDa membrane bound beta-subunit (2). Plexin B1 is a receptor for Semaphorin 4D/CD100 and it can form a receptor complex with Neuropilins, MET, and EGF R2 (4). Plexin B1 is expressed in chick retinal ganglion neurons and hippocampal neurons with a peak during the myelination period (5). Plexin B1 also plays a role in GABAergic development, changes of actin cytoskeleton, axon guidance, cell migration, and invasive growth (6, 7).

References

  1. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789
  2. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
  3. Negishi, M. et al. Cell. Mol. Life Sci. (2005) 62:1363.
  4. Franco, M. and Tamagnone, L. (2008) EMBO Rep. 9:865.
  5. Moreau-Fauvarque, C. et al. J. Neurrosci (2003) 23:9229.
  6. Swiercz, J. et al. J Cell Biol (2004) 165(6):869.
  7. Giordano, S. et al. Nat Cell Biol. (2002) 4(9):720.

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

Additional Plexin B1 Products

Product Documents for Recombinant Human Plexin B1 (Sema domain) His Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Plexin B1 (Sema domain) His Protein, CF

For research use only

Reconstitution Calculator

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