Product Specifications for Recombinant Human Plexin A1 His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human Plexin A1 protein Glu27 - Pro1244, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
130-150 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin A1 His-tag is immobilized at 2.50 µg/mL (100 µL/well), Recombinant Human Semaphorin 6C Fc Chimera (Catalog # 2219-S6) binds with an ED50 of 1.00-6.00 μg/mL.
Scientific Data Examples for Recombinant Human Plexin A1 His-tag Protein, CF
Recombinant Human Plexin A1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human Plexin A1 His-tag (Catalog # 10167-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa...
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in PBS.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
3 months, -20 to -70 °C under sterile conditions after opening.
Background: Plexin A1
Plexin A1, also known as Plexin 1, is a type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (2). Mature human Plexin A1 consists of an extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a transmembrane segment, and a large cytoplasmic domain. Within the ECD, human Plexin A1 shares 95% amino acid sequence identity with mouse and rat Plexin A1. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2 and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5-8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12). Plexin A1 has also been identified as a receptor for Slits and mediate commissural growth cone collapse (13).
Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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