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Recombinant Human Pentraxin 3/TSG-14 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10292-TS

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10292-TS-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Multimer consisting of as many as ten non-covalently and covalently linked subunits.

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Pentraxin 3/TSG-14 protein
Glu18-Ser381, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu18

Predicted Molecular Mass

41 kDa

SDS-PAGE

38-55 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human C1q is immobilized at 5 µg/mL (100 µL/well), Recombinant Human Pentraxin 3/TSF-14 His-tag (Catalog # 10292-TS) binds with an ED50 of 0.5-4 μg/mL.

Scientific Data Images for Recombinant Human Pentraxin 3/TSG-14 His-tag Protein, CF

Recombinant Human Pentraxin 3/TSG-14 His-tag Protein Binding Activity

Recombinant Human Pentraxin 3/TSG-14 His-tag Protein Binding Activity

When Human C1q is immobilized at 5 μg/mL (100 μL/well), Recombinant Human Pentraxin 3/TSG-14 His-tag (Catalog # 10292-TS) binds with an ED50 of 0.5-4 μg/mL.
Recombinant Human Pentraxin 3/TSG-14 His-tag Protein SDS-PAGE

Recombinant Human Pentraxin 3/TSG-14 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Pentraxin 3/TSG-14 His-tag (Catalog # 10292-TS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-55 kDa under reducing condition.

Formulation, Preparation and Storage

10292-TS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1-3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5). Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23-28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly upregulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG-14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

References

  1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  2. Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
  3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
  4. Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
  5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
  6. Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
  7. Introna, M. et al. (1996) Blood 87:1862.
  8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
  9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
  10. Rovere, P. et al. (2000) Blood 96:4300.
  11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
  12. Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.

Long Name

Tumor Necrosis Factor Stimulated Gene 14

Alternate Names

PTX3, TSG-14, TSG14

Entrez Gene IDs

5806 (Human); 19288 (Mouse)

Gene Symbol

PTX3

UniProt

Additional Pentraxin 3/TSG-14 Products

Product Documents for Recombinant Human Pentraxin 3/TSG-14 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Pentraxin 3/TSG-14 His-tag Protein, CF

For research use only

Reconstitution Calculator

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