Recombinant Human PDGF-BB GMP Protein, CFGMP

Name: Recombinant Human PDGF-BB GMP Protein, CF
Brand: rnd
SKU: 220-GMP

Catalog # 220-GMP

Citations (1)

Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	Q6FHE7
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source	E. coli-derived human PDGF-BB protein Ser82-Thr190 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
Purity	>97%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro
Predicted Molecular Mass	12.3 kDa (monomer)
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 1.5-6 ng/mL. The specific activity of recombinant human PDGF-BB is >7.5 x 10⁵ IU/mg, which is calibrated against the human PDGF-BB WHO International Standard (NIBSC code: 94/728).

Recombinant Human PDGF-BB GMP Protein, CF Scientific Data Examples

	Recombinant Human PDGF-BB GMP Protein Bioactivity GMP-grade Recombinant Human PDGF-BB (Catalog # 220-GMP) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED₅₀for this effect is 1.5-6 ng/mL.
	Recombinant Human PDGF-BB GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human PDGF-BB (Catalog # 220-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 26 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

220-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Item	Value
Long Name	Platelet-derived Growth Factor BB
Entrez Gene IDs	5155 (Human); 24628 (Rat)
Alternate Names	PDGF-BB, PDGFBB

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PDGF-BB GMP Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H He et al. (2017), Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 7(0):43736.
PMID: 28252047

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Recombinant Human PDGF-BB GMP Protein, CFGMP

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Key Product Details

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Product Specifications

Recombinant Human PDGF-BB GMP Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PDGF-BB

Citations (1)

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