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Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1668-ZN

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Enzyme Activity

Product Specifications


Mouse myeloma cell line, NS0-derived human Pappalysin-2/PAPP-A2 protein
Ser234-Cys1396, with a C-terminal 10-His tag


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

130 kDa


168 kDa, reducing conditions


Measured by its ability to cleave IGFBP-5.
Cleavage of Recombinant Human IGFBP‑5 (Catalog # 875-B5) by Recombinant Human Pappalysin‑2/PAPP‑A2 is detectable by SDS-PAGE stained by Coomassie, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP‑E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5 under the conditions described in Activity Assay Protocol.


  1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
  2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Long Name

Pregnancy-associated Plasma Protein

Alternate Names

PAPP-A2, PAPPA2, Pappalysin2, PAPPE, PLAC3

Entrez Gene IDs

60676 (Human)

Gene Symbol



Additional Pappalysin-2/PAPP-A2 Products

Product Documents for Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF

For research use only

Citations for Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF (4)

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  • Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/21/2022
    ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** ***Bio-Techne Technical Service provided additional support and guidance to customer developing and performing assays which differed from product QC activity specifications.***

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