Product Specifications for Recombinant Human PANK2 His-tag Protein, CF
E. coli-derived human PANK2 protein Lys206-Pro570, with C-terminal 6-His
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
Measured by its ability to transfer phosphate from ATP to pantothenic acid. The specific activity is >80 pmol/min/μg, as measured under the described conditions.
Scientific Data Examples for Recombinant Human PANK2 His-tag Protein, CF
Recombinant Human PANK2 His-tag Protein Enzyme Activity Diagram
PANK2 catalyzes the first committed step in the pathway leading to the biosynthesis of coenzyme A. PANK2 is also a key regulatory enzyme in this pathway.
Recombinant Human PANK2 His-tag SDS-PAGE.
1 μg/lane of Recombinant Human PANK2 His-tag (Catalog # 10664-PK) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 39 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
The pantothenate kinase (PANK) gene family contains four members and encode five functional isoforms. The PANK enzymes are key to the synthesis of coenzyme A (CoA) (1,2). CoA is used by around 4% of intracellular enzymes as a co-substrate. PANK2, the only member of the PANK family that resides in mitochondria, is feedback regulated by CoA and may be the master regulator of CoA synthesis (3, 4). Mutations in PANK2 results in Hallervorden-Spatz syndrome, an autosomal recessive neurodegenerative disorder caused by excessive brain iron accumulation due to the defects in CoA synthesis (1,5). The activity of this enzyme has been measured with a phosphatase-coupled method (6).
Zhou, B. et al. (2001) Nat Genet. 28:345.
Polsterm B.J. et al. (2010) Gene 465:53.
Rock, C.O. et al. (2000) J Biol Chem. 275:1377.
Srinivasan, B. et al. (2015) Nat Chem Biol. 11:784.
Orellana, D.I. et al. (2016) EMBO Mol Med. 8:1197.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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