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Recombinant Human Otolin-1 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10234-OT

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10234-OT-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Otolin-1 protein
Lys24-Pro477, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys24

Predicted Molecular Mass

48 kDa

SDS-PAGE

84-94 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PLA2G2A (Catalog # 5374-PL) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Otolin-1 His-tag (Catalog # 10234-OT) that produces 50% of the optimal binding response is 20-100 ng/mL.

Scientific Data Images for Recombinant Human Otolin-1 His-tag Protein, CF

Recombinant Human Otolin-1 His-tag Protein Binding Activity

Recombinant Human Otolin-1 His-tag Protein Binding Activity

When Recombinant Human PLA2G2A (Catalog # 5374-PL) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Otolin-1 (Catalog # 10234-OT) binds with an ED50 of 20-100 ng/mL.
Recombinant Human Otolin-1 His-tag Protein SDS-PAGE

Recombinant Human Otolin-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Otolin-1 His-tag (Catalog 10234-OT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-94 kDa and oligomers, respectively.

Formulation, Preparation and Storage

10234-OT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otolin-1

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear (1). It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function (1). It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including and Otoconin-90 (also known as PLA2G2A, PLA2L, and phospholipase A2 homolog) and Cerebellin-1 (1-3). Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus (4). It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions. Human Otolin shares 72% amino acid sequence identity with mouse and rat Otolin.

References

  1. Moreland, K.T. et al. (2014) PloS one 9:e95333.
  2. Zhao, X. et al. (2017) Dev Biol 304:508.
  3. Yang, H. et al. (2011) PLoS ONE, 6(5):e20498.
  4. Tang, Y. T. et al. (2005) Genomics 86:100.

Alternate Names

C1qTNF15, LOC131149, OTOL1

Entrez Gene IDs

131149 (Human); 229389 (Mouse); 295117 (Rat)

Gene Symbol

OTOL1

UniProt

Additional Otolin-1 Products

Product Documents for Recombinant Human Otolin-1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Otolin-1 His-tag Protein, CF

For research use only

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