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Recombinant Human Oncostatin M (HEK-293-expressed) Protein

Recombinant Human Oncostatin M (HEK-293-expressed) Protein
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With Carrier
8475-OM-01M
8475-OM-050
8475-OM-010

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Carrier Free
8475-OM-010/CF
8475-OM-050/CF

Key Product Details

Source: HEK293
Accession #: P13725
Applications: Bioactivity

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Citations (1)

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  • Recombinant Human Oncostatin M (HEK-293-expressed) Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/04/2019

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FAQs

  • What are the differences between Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM)?

    Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM) share the same protein sequence, Ala26-Arg221 of Accession # P13725, but Catalog # 295-OM was expressed in E. coli and Catalog # 8475-OM was expressed in HEK293 cells. They are offered in formulations with or without BSA as a carrier protein.Both proteins are routinely tested and have the same activity range in our established QC bioassay: "Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells." The ED50 for this effect is 0.05-0.3 ng/mL. Catalog # 8475-OM is produced in a mammalian expression system so we would expect it to be glycosylated. Catalog # 295-OM is produced in E coli and is not glycosylated.If endotoxin levels are of concern for your application, we would recommend selecting the Catalog # 295-OM as it has an endotoxin threshold of <0.01 EU per 1 μg of protein. Catalog # 8475-OM has a higher endotoxin threshold of <0.10 EU per 1 μg of the protein.  That said, if it is important to use a mammalian expressed protein like Catalog # 8475-OM in your assay system, and you require endortoxin lower than <0.10 EU/1 μg of the protein please consult technical service to see if custom testing is an option. We also recommend reviewing the references under the Citations tab on the respective product pages to check the types of applications in which these two proteins have been evaluated.

  • Is Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) considered the pre-OSM peptide or mature protein?

    Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) is comprised of amino acids Ala26-Arg221 of Accession # P13725. This region is the 196 residue mature form of OSM.

Product Datasheets

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