Recombinant Human ODC1 His-tag Protein, CF

Name: Recombinant Human ODC1 His-tag Protein, CF
Brand: R&D Systems
SKU: 10316-OD
Price: 625 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10316-OD

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Key Product Details

Source:	E. coli
Accession #:	P11926
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human ODC1 His-tag Protein, CF

Source	E. coli-derived human ODC1 protein Asn2-Val461 with an N-terminal Met and 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	52 kDa
SDS-PAGE	49 kDa, under reducing conditions
Activity	Measured by its ability to convert ornithine to putrescine. The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human ODC1 His-tag Protein, CF

	Recombinant Human ODC1 His-tag Protein Enzyme Activity Recombinant Human ODC1 His-tag Protein (Catalog # 10316-OD) is measured by its ability to convert ornithine to putrescine.
	Recombinant Human ODC1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human ODC1 His-tag (Catalog # 10316-OD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 49 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10316-OD

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: ODC1

Ornithine Decarboxylase/ODC1 is a pyridoxal phosphate (PLP)-dependent amino acid decarboxylase enzyme that catalyzes the conversion of ornithine into putrescine in a committed and rate-limiting step for polyamine synthesis. Each 51 kDa enzyme subunit contains a PLP-binding N-terminal domain and a C-terminal domain. Two active sites are formed through dimerization interfaces of the N- and C-terminal domains of opposing subunits (1). Although the enzymatically active form is homodimeric, the dimer is in rapid equilibrium due to weak association of the monomers and allows regulation of ODC1 activity through the binding of antizymes (Az) and antizyme inhibitors (AzIN) as regulatory proteins (2). ODC1 plays a crucial role in regulating polyamine levels (3) associated with cell growth, proliferation, immunity (4), and differentiation. High levels of polyamines and ODC1 are associated with cancer (5). ODC1 is a gene target of the ras and myc oncogenes (6, 7) while also capable of regulating myc expression through putrescine production (8) implicating its role as an oncogene in cancer. Mutation of ODC1 has also been shown to cause developmental delays in Bachman-Bupp syndrome (9). Consequently, ODC1 is a pharmacological target of interest (5) in a growing number of applications such as in Bachman-Bupp Syndrome (9), iron deficiency (10) and multiple cancers including osteosarcoma (11), neuroblastoma (7), breast (12), lung adenocarcinoma (13), hepatocellular carcinoma (14), and endometrial cancer (15).

Item	Value
References	Bae, D. H. et al. (2018) Biochem. Biophys. Acta. Gen. Subj.1862:2053. Wu, H. Y. et al. (2015) PNAS 112:11229. Liu, Y. C. et al. (2011) PLoS One 11:e26835. Latour, Y. L. et al. (2019) Amino Acids [epub ahead of print] (PMID 31016375). Sivashanmugam, M. et al. (2017) Biomed. Pharmacother. 86:185. Pegg, A. E. (2006) J. Biol. Chem. 281:14529. Bachmann, A. S. and D. Geerts. (2018) J. Biol. Chem. 293:18757. Tabib, A. et al. (1994) Biochem. Biophys. Res. Commun. 202:720. Bupp, C. P. et al. (2018) Am. J. Med. Genet. 176:2548. Saletta, F. et al. (2010) Mol. Pharmacol. 77:443. Weicht, R.R. et al. (2018) Med. Sci. (Basel) 6: E65. Fattahi, S. et al. (2018) Cell Mol. Biol. 64:97. Lam, S.K. et al. (2018) Oncol. Rep. 40:1994. Ye, Z. et al. (2019) Onco. Targets Ther. 12:4081. Kim, H.I. et al. (2017) PLoS One. 12:e0189044.
Long Name	Ornithine Decarboxylase 1
Entrez Gene IDs	4953 (Human); 18263 (Mouse)
Alternate Names	ODC, ODC1, ODCEC 4.1.1.17, ornithine decarboxylase, ornithine decarboxylase 1

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Recombinant Human ODC1 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human ODC1 His-tag Protein, CF

Scientific Data Examples for Recombinant Human ODC1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ODC1

Citations for Recombinant Human ODC1 His-tag Protein, CF

Product Documents for Recombinant Human ODC1 His-tag Protein, CF

Product Datasheets

COA

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