Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF New

Name: Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF
Brand: R&D Systems
SKU: 11343-NR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11343-NR

Product Datasheet / COA / SDS

Fc Chimera

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	NP_039254.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Product Specifications for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human Neuregulin-1/NRG1 protein

Human NRG1/HRG1-alpha EGF domain (Ser177-Pro226) Accession # NP_039254.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser177

Predicted Molecular Mass

32 kDa

SDS-PAGE

36-43 kDa, under reducing conditions.

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 4.00‑40.0 ng/mL.

Scientific Data Examples for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

	Recombinant Human NRG1/HRG1-alpha EGF domain Fc Chimera Protein Bioactivity. Recombinant Human NRG1/HRG1-alpha EGF domain Fc Chimera Protein (Catalog # 11343-NR) stimulates proliferation of MCF 7 human breast cancer cells. The ED₅₀ for this effect is 4.00 40.0 ng/mL.
	Recombinant Human NRG1/HRG1-alpha EGF domain Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human NRG1/HRG1-alpha EGF domain Fc Chimera Protein (Catalog # 11343-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-43 kDa and 72-86 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11343-NR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute 10.0 μg size at 100 μg/mL in PBS. Reconstitute all the other sizes at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Neuregulin-1/NRG1

Neuregulin-1 (NRG1) belongs to a family of structurally related glycoproteins encoded by four distinct but related genes, Nrg1, Nrg2, Nrg3, and Nrg4. Through alternative splicing or the use of alternative promoters, Nrg1 encodes more than 14 soluble or transmembrane proteins. Type I NRG1 isoforms include Neu Differentiation Factor, Heregulin, and ARIA. These consist of an N-terminal domain, an Ig-like domain, a linker with a Ser/Thr rich region, an EGF-like domain, a transmembrane segment, and a cytoplasmic domain. Type II isoforms such as Glial Growth Factor have a larger N-terminal domain and lack the Ser/Thr rich linker. Type III isoforms such as Sensory and Motor neuron-Derived Factor lack the Ig-like domain but contain a cysteine rich domain (CRD) and a second transmembrane segment (1 - 5). The alpha and beta splice variants of NRG1 differ in their extracellular juxtamembrane regions (3, 6). This recombinant protein corresponds to the alpha isoforms NRG1 isoforms exhibit distinct expression patterns and functions (7). The EGF-like domain, which is common to all NRG1 isoforms, is required for Neuregulin binding to ErbB3 or ErbB4 receptors (3). ErbB3 or ErbB4 subsequently heterodimerize with ErbB2, resulting in tyrosine phosphorylation and NRG1 induced signaling (1, 2). Soluble growth factors can be released by TACE/ADAM17, BACE, or ADAM19 mediated shedding of the ECD of transmembrane NRG1 (8 - 10). The cytoplasmic region can be cleaved by gamma -secretase, generating a repressor that inhibits the transcription of proapoptotic genes (11). NRG1 regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination (1, 2). In the heart, NRG1 regulates organ morphogenesis and contractility and also plays a cardioprotective role following tissue injury (12). Multiple polymorphisms and aberrant expression of NRG1 isoforms are associated with the development of schizophrenia and many cancers (1, 2, 13). Fusing Fc tag to NRG1 is reported to extend its half life in circulation and improved signal potency (14).

Item	Value
References	Mei, L. and W.-C. Xiong (2008) Nat. Rev. Neurosci. 9:437. Talmage, D.A. (2008) Novartis Found. Symp. 289:74. Holmes, W.E. et al. (1992) Science 256:1205. Marchionni, M.A. et al. (1993) Nature 362:312. Ho, W.-H. et al. (1995) J. Biol. Chem. 270:14523. Wen, D. et al. (1994) Mol. Cell. Biol. 14:1909. Meyer, D. et al. (1997) Development 124:3575. Hu, X. et al. (2006) Nat. Neurosci. 9:1520. Willem, M. et al. (2006) Science 314:664. Yokozeki, T. et al. (2007) Genes Cells 12:329. Bao, J. et al. (2003) J. Cell Biol. 161:1133. Lemmens, K. et al. (2007) Circulation 116:954. Breleux, M. (2007) Cell. Mol. Life Sci. 64:2358. Zhang, P. et al. (2018) JCI Insight. 3:e98522.
Entrez Gene IDs	3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names	ARIA, GGF2, GGFglial growth factor, HGL, HGLneu differentiation factor, HRG, HRG1, HRG1-alpha, HRG1-beta 1, HRGAneuregulin 1 type IV beta 1a, Heregulin-1, MST131, MSTP131, NDFheregulin, alpha (45kD, ERBB2 p185-activator), NRG1, Neuregulin-1, Neuregulin1, SMDF, neuregulin 1, neuregulin 1 type IV beta 3, pro-NRG1, pro-neuregulin-1, membrane-bound isoform, sensory and motor neuron derived factor

Citations for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Scientific Data Examples for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neuregulin-1/NRG1

Citations for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Product Documents for Recombinant Human NRG1/HRG1-alpha EGF domain Fc Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected