What is the half-life of this BMP protein?

Name: Recombinant Human Nodal Protein
Brand: R&D Systems
SKU: 3218-ND
Rating: 5 (2 reviews)

R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human Nodal Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3218-ND

Citations (17)

(2)

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Key Product Details

Source:	E. coli
Accession #:	Q96S42
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Nodal Protein

Product Specifications for Recombinant Human Nodal Protein

Source	E. coli-derived human Nodal protein His238-Leu347, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12.9 kDa (monomer)
Activity	Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Human Nodal can effectively induce Smad2 phosphorylation.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3218-ND

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3218-ND/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nodal

Nodal is a 13 kDa member of the TGF-beta superfamily of molecules (1-3). Its name derives from the fact that it is expressed in the primitive node of the developing embryo. In human, it is synthesized as a 347 amino acid (aa) preproprecursor that contains a 26 aa signal sequence, a 211 aa prodomain, and a 110 aa mature region (4, 5). Consistent with its TGF-beta superfamily membership, it exists as a disulfide-linked homodimer and would be expected to demonstrate a cysteine-knot motif (1, 5, 6). Mature human Nodal is 99%, 98%, 96% and 98% aa identical to mature canine, rat, bovine and mouse Nodal, respectively. Nodal signals through two receptor complexes, both of which contain members of the TGF-beta family of Ser/Thr kinase receptors. The first receptor complex is composed of ActRIB/ALK4 and ActRIIB. Nodal interaction with the ligand-binding subunit ALK4 requires the participation of fucosylated Cripto, a member of the EGF-CFC gene family. The second complex is composed of ALK7 and ActRIIB, and does not require Cripto interaction (7-9). Nodal signaling is regulated by a number of inhibitors. Nodal is reported to form nonsignaling disulfide-linked heterodimers with BMP-3, -4 and -7 (2, 6). There are also at least three secreted factors that block Nodal activation of its receptor. The first is Cerberus, a member of the DAN family of proteins. The last two are TGF-beta family members named Lefty-A and B (2, 11). The exact mechanisms employed are unclear, but Leftys are apparently induced by nodal and can work at a considerable distance from their origin (2). Nodal is known to induce both mesoderm and endoderm, participate in anterior-posterior positioning, and through ALK7, induce apoptosis in susceptible cell populations (2, 8, 12).

Item	Value
References	Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265. Schier, A.F. (2003) Annu. Rev. Cell Dev. Biol. 19:589. Whitman, M. and M. Mercola (2001) Sci. STKE. Jan 9;(64):RE1. Gebbia, M. et al. (1997) Nat. Genet. 17:305. Hart, A.H. et al. (2005) Biochem. Biophys. Res. Commun. 333:1361. Yeo, C-Y. and M. Whitman (2001) Mol. Cell 7:949. Yan, Y-T. et al. (2002) Mol. Cell. Biol. 22:4439. Munir, S. et al. (2004) J. Biol. Chem. 279:31277. Shen, M.M. and A.F. Schier (2000) Trends Genet. 16:303. Lah, M. et al. (1999) Genomics 55:364. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712. Zhou, X. et al. (1993) Nature 361:543.
Entrez Gene IDs	4838 (Human); 18119 (Mouse)
Alternate Names	BMP-16, MGC138230, Nodal, nodal homolog, nodal homolog (mouse), nodal, mouse, homolog

Citations for Recombinant Human Nodal Protein (17)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Nodal Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

J Pérez-Garc et al. (2022), Recurrent NOMO1 Gene Deletion Is a Potential Clinical Marker in Early-Onset Colorectal Cancer and Is Involved in the Regulation of Cell Migration Cancers, 14(16).
PMID: 36011023
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Harpelunde et al. (2020), Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours BMC Cancer, 20(1):349.
PMID: 32326899
Species: Simian
Sample Types: Whole Cells
Applications: Cell Culture

SC Vermilyea et al. (2020), In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets Sci Rep, 10(1):3447.
PMID: 32103062
Species: Chicken
Sample Types: Embryo
Applications: Bioassay

P Ray et al. (2018), Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 30459275
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Jørgensen et al. (2018), Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis Cell Rep, 25(7):1924-1937.e4.
PMID: 30428358
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Jiang et al. (2017), ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line Oncol Lett, 14(6):6955-6961.
PMID: 29181106
Species: Human
Sample Types: Tissue Homogenates
Applications: Bioassay

CR Ball et al. (2017), Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts EMBO Mol Med, 0(0).
PMID: 28526679
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Taroh Kinoshita (2016), A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO J. Cell Biol., 215(5):705-718.
PMID: 27881714
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Esquivies L et al. (2014), Designer nodal/BMP2 chimeras mimic nodal signaling, promote chondrogenesis, and reveal a BMP2-like structure. J Biol Chem, 289(3):1788-97.
PMID: 24311780
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Quail et al. (2014), Nodal promotes invasive phenotypes via a mitogen-activated protein kinase-dependent pathway. Oncogene, 33(4):461-73.
PMID: 23334323
Species: Human
Sample Types: Antibody
Applications: Western Blot

Fuerer C et al. (2014), Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation. J Biol Chem, 289(25):17854-71.
PMID: 24798330
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Herrera B et al. (2009), A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 10(0):20.
PMID: 19298647
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Watanabe K et al. (2007), Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. J. Biol. Chem., 282(49):35772-86.
PMID: 17925387
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

McLean AB et al. (2007), Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 25(1):29-38.
PMID: 17204604
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Watanabe M et al. (0), TGFbeta superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids. Stem Cell Report17(10):2220-2238.
PMID: 36179695

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Customer Reviews for Recombinant Human Nodal Protein (2)

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Recombinant Human Nodal Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 10/09/2020
Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 12/06/2018

Picture shows my marmoset embryonic stem cells grown with media that is supplemented with this human nodal protein. Cells are healthy, and have a homogenous morphology without spontaneous differentiation

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FAQs for Recombinant Human Nodal Protein

What is the half-life of this BMP protein?

R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human Nodal Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Nodal Protein

Formulation, Preparation and Storage

Carrier Free

3218-ND

Carrier

3218-ND/CF

Carrier Free

Reconstitution Calculator

Background: Nodal

Citations for Recombinant Human Nodal Protein (17)

Customer Reviews for Recombinant Human Nodal Protein (2)

Customer Images

Recombinant Human Nodal Protein

FAQs for Recombinant Human Nodal Protein

Product Documents for Recombinant Human Nodal Protein

Product Datasheets

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