Source	Chinese Hamster Ovary cell line, CHO-derived human NKp46/NCR1 protein Gln22-Gln254, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Gln23
Predicted Molecular Mass	27 kDa
SDS-PAGE	31-45 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human NKp46/NCR1 His-tag (Catalog # 10517-NK) is immobilized at 2.0 μg/mL, 100 μL/well, the concentration of Recombinant Human Vimentin (Catalog # 2105-VI) that produces 50% of the optimal binding response is approximately 50-500 ng/mL.

Recombinant Human NKp46/NCR1 His-tag Protein, CF Scientific Data Examples

	Recombinant Human NKp46/NCR1 His-tag Protein Binding Activity. When Recombinant Human NKp46/NCR1 His-tag (Catalog # 10517-NK) is immobilized at 2.0 μg/mL, 100 μL/well, the concentration of Recombinant Human Vimentin (2105-VI) that produces 50% of the optimal binding response is approximately 50-500 ng/mL.
	Recombinant Human NKp46/NCR1 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human NKp46/NCR1 His-tag Protein (Catalog # 10517-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 31-45 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10517-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKp46/NCR1

NKp46, also called NCR1, along with NKp30 and NKp44, are activating receptors that are collectively known as the natural cytotoxicity receptor (NCR) family (1). NKp46 is a type I transmembrane glycoprotein with two extracellular Ig-like domains followed by a short stalk region in the extracellular domain (ECD), a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. The mature ECD of human NKp46 shares 61% and 63% amino acid sequence identity with mouse and rat NKp46, respectively. NKp46 is unique among the NCRs in its evolutionary conservation between species (2). NCRs are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma (3, 4). NKp46 is involved with tumor cell recognition and has the capacity to bind to viral hemagglutinins, such as that of influenza and Sendai viruses (5, 6). NKp46 is also crucial for the development of type 1 diabetes, the clearance of Streptococcus pneumoniae, killing stellate cells in attenuated liver fibrosis, and the regulation of graft-versus-host disease and allergic response (7-10).

Item	Value
References	Hadad, U. et al. (2015) Front Immunol. 6:495. Biassoni, R. et al. (1999) Eur J Immunol. 29:1014. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197. Halfteck, G. et al. (2009) J. Immunol. 182:2221. Mendelson, M. et al. (2010) J. Virol. 84:3789. Gur, C. et al. (2011). J. Immunol. 187:3096. Elhaik-Goldman, S. et al. (2011). PLoS One. 6:e23472. Gur, C. et al. (2012) Gut. 61:885. Ghadially, H. et al. (2013) Eur. J. Immunol. 43:3006.
Entrez Gene IDs	9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names	CD335, CD335 antigen, LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46), Ly94, Lymphocyte antigen 94 homolog, MAR-1, NCR1, NK cell-activating receptor, NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46), NKP46FLJ99094, NKp46, Natural killer cell p46-related protein, hNKp46, natural cytotoxicity triggering receptor 1

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Recombinant Human NKp46/NCR1 His-tag Protein, CF

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