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Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Bio-Techne includes R&D Systems | Catalog # 367-NR

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Carrier Free
367-NR-050/CF

Catalog #
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With Carrier
367-NR-050

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NGF R/TNFRSF16 protein
Human NGF R
Lys29-Asn250
Accession # P08138
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys29

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.2-0.6 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 367-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 367-NR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFR/TNFRSF16

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

References

  1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
  2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
  3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Long Name

Nerve Growth Factor Receptor

Alternate Names

CD271, NGF R, p75 NTR, TNFRSF16

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Gene Symbol

NGFR

UniProt

Additional NGFR/TNFRSF16 Products

Product Documents for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

For research use only

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