Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Name: Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein
Brand: R&D Systems
SKU: 367-NR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 367-NR

Citations (8)

Product Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P08138
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Product Specifications for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NGF R/TNFRSF16 protein

Human NGF R Lys29-Asn250 Accession # P08138	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Lys29

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit beta -NGF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.2-0.6 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

367-NR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

367-NR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NGFR/TNFRSF16

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

Item	Value
References	Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Long Name	Nerve Growth Factor Receptor
Entrez Gene IDs	4804 (Human); 18053 (Mouse); 117089 (Rat)
Alternate Names	CD271, CD271 antigen, Gp80-LNGFR, NGF R, NGF receptor, NGFR, TNFRSF16, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), member 16, nerve growth factor receptor, p75 ICD, p75 NTR, tumor necrosis factor receptor superfamily member 16

Citations for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein include:

Species: Human
Sample Types: Urine
Applications: ELISA (Standard)

SR Shepheard et al. (2017), Urinary p75(ECD): A prognostic, disease progression, and pharmacodynamic biomarker in ALS Neurology, 88(12):1137-1143.
PMID: 28228570
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Adams et al. (2017), Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling Sci Rep, 7(0):37716.
PMID: 28134246
Species: Human
Sample Types: Urine
Applications: ELISA (Standard)

R Jia et al. (2017), Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort Sci Rep, 7(1):5127.
PMID: 28698670
Species: Human
Sample Types: Protein
Applications: Bioassay

A Suzuki et al. (2017), Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth Biol. Pharm. Bull., 40(10):1784-1788.
PMID: 28966252
Species: Human
Sample Types: Urine
Applications: ELISA (Standard)

Shepheard S et al. (2014), The extracellular domain of neurotrophin receptor p75 as a candidate biomarker for amyotrophic lateral sclerosis. PLoS ONE, 9(1):e87398.
PMID: 24475283
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Fobian K et al. (2010), Peptides derived from the solvent-exposed loops 3 and 4 of BDNF bind TrkB and p75(NTR) receptors and stimulate neurite outgrowth and survival. J. Neurosci. Res., 88(6):1170-81.
PMID: 19908279
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Lu B et al. (2008), A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 76(8):3530-8.
PMID: 18541656
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Song XY et al. (2004), Suppression of p75NTR does not promote regeneration of injured spinal cord in mice. J. Neurosci., 24(2):542-6.
PMID: 14724254

Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Formulation, Preparation and Storage

Carrier Free

367-NR

Carrier

367-NR/CF

Carrier Free

Reconstitution Calculator

Background: NGFR/TNFRSF16

Citations for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein (8)

Product Documents for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

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COA

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SDS

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