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Recombinant Human Neuropilin-1 Fc Chimera Protein, CF

Catalog # 10455-N1 | R&D Systems, Inc. a Bio-Techne Brand
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10455-N1-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Neuropilin-1 protein
Human Neuropilin-1
(Phe22-Lys852)
Accession # NP_003864.4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

120 kDa

SDS-PAGE

117-131 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Neuripilin-1 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human VEGF 165 (Catalog # 293-VE) binds with an ED50 of 0.25-1.5 ng/mL.

Scientific Data Images for Recombinant Human Neuropilin-1 Fc Chimera Protein, CF

Recombinant Human Neuropilin-1 Fc Chimera Protein Binding Activity.

When Recombinant Human Neuropilin-1 Fc Chimera (Catalog # 10445-N1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human VEGF 165 (Catalog # 293-VE) binds with an ED50 of 0.25-1.5 ng/mL.

Recombinant Human Neuropilin-1 Fc Chimera Protein SDS-PAGE.

2 µg/lane of Recombinant Human Neuropilin-1 Fc Chimera (Catalog # 10455-N1) was resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 117-131 kDa and 220-250 kDa, respectively.

Formulation, Preparation and Storage

10455-N1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, also known as neuropilin and CD304) is a 130-140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1-4). The full-length 923 amino acid (aa) human Npn-1 isoform 1 contains an 835 aa extracellular domain (ECD) that shows 92-95% aa identity with mouse, rat, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5-7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF 165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF 165 binding (1). Sema3A signaling can be blocked by VEGF 165, which has higher affinity for Npn-1 (12). Npn-1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13). Npn-1 is a marker of CD4+ Treg cells and a population of CD8+ T-cells infiltrating solid tumors. Immunotherapies that block Npn-1 synergizes with anti-PD-1 to enhance CD8+ proliferation and response (14). There is evidence that Npn-1 with VEGF-A plays a role in stemness of breast cancer cell by activating Wnt/b-catenin pathway (15).

References

  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
  2. Gu, C. et al. (2003) Dev. Cell 5:45.
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735.
  5. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573.
  6. Cackowski, F.C. et al. (2004) Genomics 84:82.
  7. Rossignol, M. et al. (2000) Genomics 70:211.
  8. Tao, Q. et al. (2003) Angiogenesis 6:39.
  9. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
  10. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
  11. Yaron, A. et al. (2005) Neuron 45:513.
  12. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
  13. Tordjman, R. et al. (2002) Nat. Immunol. 3:477.
  14. Leclerc, M. et al. (2019) Nat Commun. 10:3345.
  15. Zhang, L. et al. (2017) Cell Physiol. Biochem. 44:1251.

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

UniProt

Additional Neuropilin-1 Products

Product Documents for Recombinant Human Neuropilin-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Neuropilin-1 Fc Chimera Protein, CF

For research use only

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